Karius at
ASM Microbe 2020
Scientific Abstracts
Non-Invasive Detection of Pathogens
Validation of the Karius Test
Rapid, Liquid Biopsy of Kingella kingae Vertebral Infections in Infants Using a Plasma-based Microbial Cell-free DNA Sequencing Test for Pathogen Detection
Rapid, Non-invasive Detection of Legionella and Resolution of Species Diversity in Clinical Infections Using a Plasma-based Microbial Cell-free DNA Sequencing Test for Pathogen Identification
Successful Detection of Unrecognized Rickettsia typhi in Pregnancy Using Cell Free Next-generation Sequencing
Impact of Next Generation Sequencing vs. Conventional Microbiologic Tests of Bronchoscopic Samples on Patient Care
Analytical Validation of a Culture-free Antimicrobial Resistance Test Based on Agnostic Microbial Cell-free DNA Sequencing
Rapid, Liquid Biopsy of Kingella kingae Vertebral Infections in Infants Using a Plasma-based Microbial Cell-free DNA Sequencing Test for Pathogen Detection
Rapid, Non-invasive Detection of Legionella and Resolution of Species Diversity in Clinical Infections Using a Plasma-based Microbial Cell-free DNA Sequencing Test for Pathogen Identification
Impact of Next Generation Sequencing vs. Conventional Microbiologic Tests of Bronchoscopic Samples on Patient Care
Analytical Validation of a Culture-free Antimicrobial Resistance Test Based on Agnostic Microbial Cell-free DNA Sequencing
Successful Detection of Unrecognized Rickettsia typhi in Pregnancy Using Cell Free Next-generation Sequencing
Rapid, Liquid Biopsy of Kingella kingae Vertebral Infections in Infants Using a Plasma-based Microbial Cell-free DNA Sequencing Test for Pathogen Detection
A. Sarmiento¹, N. Kabani², A. Del Valle Penella¹, M. Laufer¹, C. Sanchez-Vegas¹, M. Cotilla¹, M. Melish³, C. Trieu², J. C. Arnold⁴, A. Jordan Villegas⁵, D. Kimberlin², M. S. Lindner⁶, A. A. Ahmed⁶
¹Nicklaus Children's Hosp., Miami, FL, ²Children's of Alabama & The Univ. of Alabama at Birmingham, Birmingham, AL, ³John A. Burns Sch. of Med., Univ. of Hawai'i, Honolulu, HI, ⁴Naval Med. Ctr. San Diego, San Diego, CA, ⁵Arnold Palmer Hosp. For Children, Orlando, FL, ⁶Karius, Redwood City, CA
Summary The Karius Test showed that antibiotics were narrowed in 83% (5/6) of cases while enabling a diagnosis without invasive biopsy in 100% (6/6) of cases.
Session CIV05 Pediatric Infectious Diseases
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Impact of Next Generation Sequencing vs. Conventional Microbiologic Tests of Bronchoscopic Samples on Patient Care
P. Mead, H. Shoaib, R. Davis, J. Farrell
Session CPHM12 Cell Free DNA and Sequencing Approaches
Summary In a retrospective cohort of 172 patients, the impact factor of the KT was more than double (7.8 vs. 3.3) the impact factor of bronchoscopy for patients with suspected invasive pulmonary infections.
Univ. of Illinois Coll. of Med. at Peoria, Peoria, IL
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Rapid, Non-invasive Detection of Legionella and Resolution of Species Diversity in Clinical Infections Using a Plasma-based Microbial Cell-free DNA Sequencing Test for Pathogen Identification
A. A. Ahmed, S. C. Dalai, L. Blair, M. S. Lindner, A. MacIntyre
Session CPHM12 Cell Free DNA and Sequencing Approaches
Summary In 29 patient samples, the Karius Test was able to identify L. pneumophila and less-commonly detected non-pneumophila Legionella species including: Legionella anisa, Legionella bozemanae, Legionella micdadei.
Karius, Redwood City, CA
Analytical Validation of a Culture-free Antimicrobial Resistance Test Based on Agnostic Microbial Cell-free DNA Sequencing
S. Venkatasubrahmanyam, L. Blair, M. Rosen, M. Borivojevic, L. Guz, M. Lindner, D. Hong, A. Ahmed, D. Hollemon, C. Ho, J. Wilber, S. Bercovici
Session CPHM12 Molecular Susceptibility Testing
Summary Investigation of microbial cell-free DNA for the detection of methicillin-resistant and -sensitive Staphylococcus aureus (MRSA and MSSA) shows a culture-independent method for detecting AMR directly from blood.
Karius, Redwood City, CA
Successful Detection of Unrecognized Rickettsia typhi in Pregnancy Using Cell Free Next-generation Sequencing
I. A. Stafford, F. Hernandez-Centero, G. Parkerson, M. Al Mohajer, L. Woc-Colburn, A. J. Burgos Lee, M. Rac, J. Dunn, K. Muldrew
Session CPHM12 Cell Free DNA and Sequencing Approaches
Summary The Karius Test accurately and rapidly identified R. typhi when initial blood, urine, and stool cultures were negative for pathogens. This enabled targeted and prompt therapy.
Baylor Coll. of Med., Houston, TX
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Non-Invasive Detection of Kingella kingae Vertebral Infections Using the Karius Test
A. Sarmiento et al. Rapid, Liquid Biopsy of Kingella kingae Vertebral Infections in Infants Using a Plasma-based Microbial Cell-free DNA Sequencing Test for Pathogen Detection. Presented at ASM Microbe Online 2020. June 22, 2020 - August 17, 2020.
A case review of seven cases of Kingella kingae pediatric vertebral infections across five institutions.
Case Presentations on Vertebral Infections
The study on the clinical and analytical validation of the Karius Test was published in Nature Microbiology.
This study addressed the challenges of bringing a metagenomic infectious disease diagnostic test to clinicians.
Blauwkamp, et al. Nat Microbiol 2019 April, 4(4):663-674.
Analytical Sensitivity
>95%
at 50 fragments of
cell-free DNA per µL plasma
Analytical Specificity
Per Sample
>98%
49 out of 50 true
negative samples
Analytical Specificity
Per Organism
99.998%
62,449 out of 62,450 expected negative
Read the Validation Study
Rapid, Liquid Biopsy of Kingella kingae Vertebral Infections in Infants Using a Plasma-based Microbial Cell-free DNA Sequencing Test for Pathogen Detection
Rapid, Non-invasive Detection of Legionella and Resolution of Species Diversity in Clinical Infections Using a Plasma-based Microbial Cell-free DNA Sequencing Test for Pathogen Identification
Impact of Next Generation Sequencing vs. Conventional Microbiologic Tests of Bronchoscopic Samples on Patient Care
Analytical Validation of a Culture-free Antimicrobial Resistance Test Based on Agnostic Microbial Cell-free DNA Sequencing
Successful Detection of Unrecognized Rickettsia typhi in Pregnancy Using Cell Free Next-generation Sequencing
Rapid, Liquid Biopsy of Kingella kingae Vertebral Infections in Infants Using a Plasma-based Microbial Cell-free DNA Sequencing Test for Pathogen Detection
A. Sarmiento¹, N. Kabani², A. Del Valle Penella¹, M. Laufer¹, C. Sanchez-Vegas¹, M. Cotilla¹, M. Melish³, C. Trieu², J. C. Arnold⁴, A. Jordan Villegas⁵, D. Kimberlin², M. S. Lindner⁶, A. A. Ahmed⁶
Session CIV05 Pediatric Infectious Diseases
Summary The Karius Test showed that antibiotics were narrowed in 83% (5/6) of cases while enabling a diagnosis without invasive biopsy in 100% (6/6) of cases.
¹Nicklaus Children's Hosp., Miami, FL, ²Children's of Alabama & The Univ. of Alabama at Birmingham, Birmingham, AL, ³John A. Burns Sch. of Med., Univ. of Hawai'i, Honolulu, HI, ⁴Naval Med. Ctr. San Diego, San Diego, CA, ⁵Arnold Palmer Hosp. For Children, Orlando, FL, ⁶Karius, Redwood City, CA
Impact of Next Generation Sequencing vs. Conventional Microbiologic Tests of Bronchoscopic Samples on Patient Care
P. Mead, H. Shoaib, R. Davis, J. Farrell
Session CPHM12 Cell Free DNA and Sequencing Approaches
Summary In a retrospective cohort of 172 patients, the impact factor of the KT was more than double (7.8 vs. 3.3) the impact factor of bronchoscopy for patients with suspected invasive pulmonary infections.
Univ. of Illinois Coll. of Med. at Peoria, Peoria, IL
Read Abstract
Rapid, Non-invasive Detection of Legionella and Resolution of Species Diversity in Clinical Infections Using a Plasma-based Microbial Cell-free DNA Sequencing Test for Pathogen Identification
A. A. Ahmed, S. C. Dalai, L. Blair, M. S. Lindner, A. MacIntyre
Session CPHM12 Cell Free DNA and Sequencing Approaches
Summary In 29 patient samples, the Karius Test was able to identify L. pneumophila and less-commonly detected non-pneumophila Legionella species including: Legionella anisa, Legionella bozemanae, Legionella micdadei.
Karius, Redwood City, CA
Analytical Validation of a Culture-free Antimicrobial Resistance Test Based on Agnostic Microbial Cell-free DNA Sequencing
S. Venkatasubrahmanyam, L. Blair, M. Rosen, M. Borivojevic, L. Guz, M. Lindner, D. Hong, A. Ahmed, D. Hollemon, C. Ho, J. Wilber, S. Bercovici
Session CPHM12 Molecular Susceptibility Testing
Summary Investigation of microbial cell-free DNA for the detection of methicillin-resistant and -sensitive Staphylococcus aureus (MRSA and MSSA) shows a culture-independent method for detecting AMR directly from blood.
Karius, Redwood City, CA
Successful Detection of Unrecognized Rickettsia typhi in Pregnancy Using Cell Free Next-generation Sequencing
I. A. Stafford, F. Hernandez-Centero, G. Parkerson, M. Al Mohajer, L. Woc-Colburn, A. J. Burgos Lee, M. Rac, J. Dunn, K. Muldrew
Session CPHM12 Cell Free DNA and Sequencing Approaches
Summary The Karius Test accurately and rapidly identified R. typhi when initial blood, urine, and stool cultures were negative for pathogens. This enabled targeted and prompt therapy.
Baylor Coll. of Med., Houston, TX
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Scientific Abstracts
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Scientific Abstracts
Non-Invasive Detection
Validation of the Karius Test
Patient 1
Patient 2
Patient 3
Patient 4
Patient 5
Demographic data was available for seven cases, clinical data was available for six.
Blood culture result
Empiric antibiotics
Antibiotic pretreatment
prior to Karius Test
Impact of Karius Test results on treatment management
Non-invasive diagnosis of Kingella kingae vertebral infection without biopsy
Outcome
Clindamycin
Clindamycin
Vancomycin
Ceftriaxone
Vancomycin
Ceftriaxone
Vancomycin
Ceftriaxone
None
None
1 day
2 days
2 days
Narrow to ceftriaxone
Narrow to ceftriaxone
No
impact
Narrow to ceftriaxone (cephalexin oral)
Narrow to cefazolin
Full recovery
Full recovery
Full recovery
Recovery, with mild spinal height loss
Full recovery
®
Negative blood cultures in 100% (6/6) of cases
Non-invasive diagnosis without biopsy
100%
(6/6)
Karius Test results had impact on treatment in 83% (5/6) cases
Patient 6
Clindamycin
Ceftriaxone
Full recovery
None
Narrow to ceftriaxone (cephalexin oral)
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