Deeper Insights for Every Lab
The ImageXpress HCS.ai High-Content Screening System with AI-powered Insights
LED or LASER ILLUMINATION
CONFOCAL SPINNING DISK
CUSTOM OPTICS 6 objective turret Water immersion objectives
SCIENTIFIC CMOS SENSOR
MAGNIFICATION CHANGER
AgileOptix™ technology offers a combination of a powerful solid-state light engine, custom optics, scientific CMOS sensor, and the ability to change between different disk geometries.
Cell painting image at 60X magnification captured under high-intensity laser illumination
LED light source Gentle illumination compatible with up to 7 fluorescent channels: DAPI, CFP, FITC, YFP, TRITC, TxRED, Cy5 Ideal for widefield, confocal and standard screening assays 7-Laser high-intensity light source Powerful excitation compatible with up to 8 fluorescent channels Including near infra-red illumination for wider spectral separation and more fluorophore options DAPI, CFP, FITC, YFP, TRITC, TxRED, Cy5, Cy7 Boost acquisition speeds with significantly reduced exposure times, ideal for 3D and highly multiplexed assays
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Organoid image acquired with 50µm pinhole deep tissue disk
Organoid image acquired with 60µm pinhole confocal disk
Multiple confocal disk geometries, including pinhole and slit configurations for high-throughput applications. Standard disk (single): 60μm pinhole disk - great for wide range of 2D and 3D applications Screening disk (dual): 60μm pinhole paired with 50μm slit disk - optimized for fast screening speeds Deep tissue disk (dual): 50μm pinhole disks with smaller pinholes and spacing optimized for higher signal or deep sectioning in 3D structures
Proprietary AgileOptix™ Spinning Disk Technology
Live-cell time-lapse images acquired using water immersion objectives at 10X
Equipped with up to six objectives at any one time, with magnifications from 2X to 60X, including high NA and ELWD objectives, reducing the need for configuration changes. The system can also be equipped with up to 2 water immersion objectives. These offer signal increases of up to 4X, helping scientists see deeper into 3D and thick tissue samples with lower exposure times.
6-Position automated objective changer with optional water immersion
PC-12 cells captured at 40X magnification
The ImageXpress HCS.ai system can be equipped with a magnification changer that can switch between 1X and 1.5X. This allows up to 12 magnifications in a single configuration without the need to switch objectives.
Automated magnification changer
HCT116 spheroid (cleared) imaged with 20x Water Immersion
A >5 mega pixel sCMOS sensor with large sensor size (224mm2) and a >3 log dynamic range with 16bit detector offers 95% peak quantum efficiency – enabling you to get clearer images faster.
sCMOS Sensor with exceptional sensitivity and wide field of view
The updated MetaXpress® Acquire High-Content Image Acquisition Software streamlines complex workflows. New users can get started quickly thanks to the intuitive, redesigned interface and guided workflows, whereas advanced users can leverage its flexibility to create optimized protocols.
Intuitive image acquisition software for a wide range of applications – optimized for the ImageXpress HCS.ai system
MetaXpress Acquire High-Content Image Acquisition Software
6-channel cell painting acquisition in MetaXpress Acquire software
IN Carta® image analysis software's advanced features provide the functionality you need to analyze data in 2D, 3D, and 4D at scale and deliver real-time insights without the need for complex pre- or post- processing operations. Improve the specificity of your image analysis workflows by utilizing the SINAP (Segmentation Is Not A Problem) deep-learning module. Put machine learning to work for you and perform complex phenotypic analysis within a user-friendly Phenoglyphs module.
AI-powered analytics combined with an intuitive user interface.
IN Carta Image Analysis Software
IN Carta 3D View - spheroid interaction
QuickID Targeted Acquisition works by acquiring an entire field-of-view of a sample at low magnification, finding objects of interest or rare events through a fast image analysis process, and then re-acquiring only these wells or sites at higher magnification with a user-configured protocol that may include the relevant wavelengths, z-stacks, and time-points. This results in faster image acquisition, fewer images to analyze, and less data storage space required – as well as ensuring the object of interest is always centered.
QuickID™ Targeted Image Acquisition
Train your system to recognize objects of interest with IN Carta deep-learning segmentation.
