ImageXpress Pico Automated Cell Imager Feature Update
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Automated Focus Routines
Hardware autofocus uses an LED beam to find reflective surfaces and is designed for speed. It works well for adherent samples in plates or chamber slides. When enabled, image-based autofocus searches a range for the best focus plane based on image contrast. It works well for slides with a coverslip or for samples in a plate that are not flat, such as suspension cells or spheroids.
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Detect Surface Algorithms
Find Best Plane Algorithms
Anchor Focus Position
Hardware autofocus detects the surface closest to the objective (that is, the plate bottom). It works well for adherent samples in plates or chamber slides when imaging at low magnification or if your labware has a thick bottom. This is the fastest hardware autofocus option.
Detect Surface Algorithms - Plate Bottom
Hardware autofocus detects the two surfaces closest to the objective (that is, the plate bottom and well bottom). The Well Bottom option is designed for samples in a liquid medium, such as well plates or chamber slides. This is the most commonly used hardware autofocus option.
Detect Surface Algorithms - Well Bottom
Hardware autofocus detects the three surfaces closest to the objective (that is, the plate bottom, well bottom, and well insert). The Well Insert option is designed for well inserts in well plates or any labware design that has a distinct third surface.
Detect Surface Algorithms - Well Insert
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The Find Best Plane algorithms center on the surface detected by the hardware autofocus and search within a specific range to find the optimal focal plane.
Superwide Search Range
Uses the surface selected by the hardware autofocus and searches a range of 300 µm above the surface (unlike the other options, which search around the surface)
Wide Search Range
Centered on the surface selected by the hardware autofocus. It searches a range of 40% of the Bottom Thickness value from the labware specification (20% above the surface and 20% below it)
Normal Search Range
Centered on the surface selected by the hardware autofocus. It searches a range around the surface of 15% of the Bottom Thickness value from the labware specification (7.5% above the surface and 7.5% below it)
For fastest screening speed, we have introduced the ability to select the Anchor Focus Position to save current focus position and disable the autofocus controls. The software uses the saved focus position for any ensuing snaps of preview images and for acquisition. This can dramatically speed the acquisition of plates at low magnification or when you are working with macroscopic samples like whole organisms or tissues.
Press ESC to exit full screen
Plate Bottom
Well Bottom
Digital Confocal
Digital Confocal 2D on-the-fly deconvolution
Standard Widefield Imaging
Toggle Digital Confocal
Significantly increase resolution and assay quality with Digital Confocal 2D on-the-fly deconvolution. The Digital Confocal Option restores light to its original point of origin, allowing you to decrease exposure time and improve statistical significance of your observations. Digital Confocal is seamlessly integrated into Pico’s fluorescent image acquisition workflow allowing you to capture fewer z-stacks, meaning faster scan speeds, with higher signal to noise data for more precise segmentation and analysis.
Click to toggle Digital Confocal on/off
Live Preview
Simplify the identification of ROIs (regions of interest). Visualize your sample prior to acquisition using the virtual joystick to pan around the sample, and interactively adjust focus. Live Preview, featuring “click-to-center” functionality, continuously updates the image so you can easily navigate your sample to find the desired field of view. Whether you’re working on 96-well microplates, slides, or 35mm culture dishes, Live Preview helps you to quickly and easily focus in on what’s important to your research.
Click to view Live Preview
Activate "Live Preview"
Save Position
Save Offset
Click to center image
Image centered
Change magnification
Run multi-color acquisition
Click to toggle Environmental Control
Environmental Control
Environmental control mimics the cell environment and enables you to run multiday studies, time-lapse, and live cell assays. With CellReporterXpress™ software, the ImageXpress® Pico Automated Cell Imaging System provides visibility of environmental control settings for humidity, Carbon Dioxide, and Oxygen during acquisition to ensure that the system is running at peak performance during your assay.
The environmental control system integrates directly into the rear of the ImageXpress Pico saving precious lab bench space. Easily add water without having to disrupt your ongoing experiments to support multiday studies.
The unique magnetically sealed environmental control cassette chamber introduces and maintains gas and humidity while reducing gas usage as compared to flooding the entire system.
The ImageXpress Pico with CellReporterXpress Image Acquisition and Analysis Software features Multi-Wavelength Cell Scoring with up to four fluorescent stains. The pre-configured protocol is ideal for counting and logging measurements of cells in multiple wavelength experiments. Using a fluorescent marker for the nucleus and additional markers for the cytoplasm, each wavelength is analyzed and cells are assigned multiparametric phenotypic profiles.
Click to toggle Cell Scoring
Multi-Wavelength Cell Scoring
A simple interface minimizes setup efforts and analysis settings can be configured once and saved for future use or customized to fit a specific experiment. Segmentation parameters are set for each wavelength and the analysis is run across the well, selected wells, the entire plate or multiple plates.
Go from samples to results in minutes
There are a wide variety of data visualization tools available from the plate to the cellular level in the CellReporterXpress software empowering users to learn as much as possible from their images and assays. Each user has the ability to export cell level data in addition to plate level data at the same time, from the same analysis.
Click to toggle Data Visualization
Data Visualization Tool
Easily view trends across your assay with the plate Thumbnail View. From this view, you can double-click on a well to open the full resolution image for that well, select a group of wells to visualize, or you can open and review every image that was captured.
Thumbnail View
There are various data visualization tools to review plate level data, and if desired, filter down to cellular level data based upon specific readouts from your analyses. For example, in the plate level Scatter Plot, highlight data points (wells) to gate populations of cells based on well-by-well data. This will open up access to the Cellular Data Visualization Tools.
Plate Level Scatter Plot
Open up Cell Level Density Heatmap to visualize a Scatter Plot of cell-by-cell data. Each data point is an individual cell, and the X and Y axes, as well as the heatmap, can be configured to display your desired cellular measurements from your analysis. Gate further by highlighting a group of cells.
Cell Level Density Heatmap
Once you’ve gated your cells of interest in the previously mentioned Cellular Data Visualization Tools, open up the high-resolution images of these cells with the Cell Level Images icon. Individual cells will be highlighted with a green box.
Cell Level Images and Cell Info Mode
CellReporterXpress software provides you with the ability to export the entirety of your multiparametric cell level data, while also enabling you to filter and sort out specific sub-populations of cells that express a phenotype of interest. These sub-populations can then be visualized in an interactive interface and the relevant measurements for these cells can be exported exclusively.
Look at the data analysis measurements for each highlighted cell by selecting the Cell Info Mode icon and clicking on the highlighted cell outlined in green.
Toggle through the images of all the gated cells within the Image Gallery. The white images are the image analysis segmentation masks of the cells. Each cell has a unique identifier (well name_number).
From samples to results in minutes. The ImageXpress Pico system features over 25 preconfigured templates optimized to collect the most pertinent information for various cell-based assays removing the guesswork from optimizing parameters. User guided artificial intelligence routines optimize parameters automatically resulting in robust, easy-to-use analysis protocols.
Click to view applications
Applications
Angiogensis
Apoptosis
Autophagy
Cell Counting
Cell Count - Transmitted Light
Cell Differentiation
Endocytosis
Internalization
Live Cells
Cell Scoring
Cell Scoring - Transmitted Light
Double Marker Expression
Multi-Color Cell Scoring
Neurite Tracing
Phagocytosis
Lysosomes
Mitochondria
Mitotic Index
Viral Infectivity
Custom Mimetas 2 Lane Protocol
Pits and Vessicles
Protein Expression Index
Translocation
Custom Mimetas 3 Lane Protocol
Slide Colorimetrics Acquisition
Stitched Slide Colorimetrics
Validation Test Bead Plate
Triple Marker Expression
Plate Colorimetric Acquisition
Stitched Plate Colorimetrics
Validation Test Colorimetric Slide
Custom Labware Protocols
The ImageXpress Pico system accommodates various labware options including slide holders, 35mm culture dish holders, and more.
Click to view Labware
Additional Labware Support
4 Slide Holder
Magnetically sealed environmental control cassette
35mm culture dish holder
Transwell Plates
6- to 384-Well Plates
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