Media Exchange
Monitoring
Automated Passaging & Hydrogel Dome Dissociation
Centrifugation
Replating
Confocal Imaging and Analysis
ImageXpress HCS.ai
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Scientific Poster
AI-enabled automated compound screening for toxicity effects using healthy intestinal 3D organoids
Scientific Poster
AI-enabled automated compound screening for toxicity effects using healthy intestinal 3D organoids
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AI-enabled automated compound screening for toxicity effects using healthy human intestinal organoids
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AI-enabled automated compound screening for toxicity effects using healthy human intestinal organoids
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Cell culture automation of the 3D cancer spheroid assay with the CellXpress.ai cell culture system
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Cell culture automation of the 3D cancer spheroid assay with the CellXpress.ai cell culture system
Scientific Poster
Machine learning-assisted automation of complex 2D and 3D cell culture and assay models
Scientific Poster
Machine learning-assisted automation of complex 2D and 3D cell culture and assay models
X, Y coordinate of automatic Matrigel dome placement across multiple plates and wells for multiple passaging cycles.
Custom Organoid Expansion Service
Publication
Custom Organoid Expansion Service
Publication
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Daily imaging-based experimental monitoring provides at-a-glance information about the status of your cell culture. Track growth curves and morphology to achieve in-experiment insights into your culture health. Utilize automated machine-learning based decision making to analyze cell density and morphology to determine optimal passaging timing or utilize data visualization to supports quick manual decision-making.
Monitoring
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Automated scheduling and feeding maintains optimal cell growth conditions and leads to enhanced cell health and experimental consistency. Never miss a weekend or holiday feeding since the CellXpress.ai Automated Cell Culture System works weekends so you don't have to.
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Media Exchange
The first step in culturing intestinal organoids is seeding or plating in this case in hydrogel domes. The CellXpress.ai Automated Cell Culture System plates cells with precision minimizing both volume and dome placement variability. Consistent seeding volume is an important element in cell culture growth as it leads to well-to-well consistency of growth curves. Consistent dome placement leads to consistent imaging: no need to hunt around the well for the dome.
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The CellXpress.ai system automates dome dissociation, ensuring gentle and reproducible passaging is done at the optimal time and with without manual intervention. The CellXpress.ai automates the mixing and pipette movement to disassociate hydrogel domes as part of the passaging step. Automated protocol execution handles the complete passaging workflow, from media removal to cell detachment and collection. Standardized parameters ensure consistent application of dissociation agents and incubation times to eliminate process variability. By automating the passaging process, the subjectivity of determining when to passage is eliminated.
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Automated Passaging and Hydrogel Dome Dissociation
Automated centrifugation separates extracellular matrix (ECM) from organoid fragments. Automated plate handling and centrifugation ensures clean ECM separation and removal prior to replating to support high-throughput workflows and reliable results. Avoid workflow interruptions by automating the passaging step.
Centrifugation
Whether you are performing 1:1 passaging or scaling up, the CellXpress.ai system streamlines complex cell culture steps to accelerate discovery. By performing all tasks necessary for cell culture including cell plating, media exchanges, passaging, monitoring by imaging, and automated decision making, the CellXpress.ai system gives you control over demanding cell culture feeding and passaging schedules—eliminating time in the lab while maintaining a 24/7 schedule for growing and scaling multiple stem cell lines, spheroids, or organoids.
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Replating
Put machine learning to work for you to improve the specificity of your image analysis workflows with deep learning-based phenotypic classification to efficiently evaluate assays.
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Confocal Imaging and Analysis
Cell Plating in Hydrogel Domes
The CellXpress.ai Automated Cell Culture System plates cells with precision minimizing both volume and dome placement variability
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Never miss a weekend or holiday feeding since the CellXpress.ai Automated Cell Culture System works weekends so you don't have to.
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Daily imaging-based experimental monitoring provides at-a-glance information about the status of your cell culture.
The CellXpress.ai system automates dome dissociation, ensuring gentle and reproducible passaging is done at the optimal time.
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Automated plate handling and centrifugation ensures clean ECM separation and removal prior to replating.
Whether you are performing 1:1 passaging or scaling up, the CellXpress.ai system streamlines complex cell culture steps to accelerate discovery.
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AI-powered analytics combined with an intuitive user interface provide the functionality you need to analyze data at scale and deliver real-time insights without the need for complex pre- or post- processing operations.
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Cell Plating in
Hydrogel Domes
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Cell Plating in
Hydrogel Domes
Media Exchange
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Monitoring
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Automated Passaging & Hydrogel Dome Dissociation
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Centrifugation
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Replating
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Confocal Imaging and Analysis
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Give your lab the confidence in experimental outcomes to make key decisions sooner and achieve milestones faster with fewer failed experiments.
Built by scientists, for scientists, the CellXpress.ai system features AI-driven decision-making and biologist-friendly protocols that automate 2D and 3D model cell culture processes to eliminate manual intervention, streamline workflows, and reduce hands-on time in the lab.
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The ImageXpress HCS.ai system is built with a modular architecture that grows with your research. Whether you're starting with widefield imaging or advancing into confocal and deep tissue analysis, this system evolves with your needs—no full system replacement required. A broad range of configuration options and intuitive acquisition software with AI and machine-learning capabilities make it easy to upgrade, adapt, and scale—future-proofing your investment and accelerating discovery.
Deeper insights for every lab
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Cell plating in
hydrogel domes
Media exchange
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Monitoring
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Disruption of domes
and organoids
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Centrifugation
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Mix cells with
hydrogel, re-plate
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Confocal imaging, image analysis
Centrifuge
CellXpress.ai
Automated
Cell Culture
System
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Intestinal Organoid Workflow