4X
NA 0.13
10X
NA 0.32
20X
NA 0.40
40X
NA 0.60
63X
NA 0.70
ImageXpress Pico utilizes user-exchangeable HC PL Fluotar air objectives with extra long working distances by Leica Microsystems on a 6 position automated turret.
4X Leica PL Fluotar objective (NA 0.13)
Ideal for slides, low-well number plates (6-well, 12-well plates), and whole-well imaging.
PL Fluotars are powerful universal objectives with outstanding chromatic correction for at least three wavelengths which makes them suitable for fluorescence imaging.
Field planarity (PL) is computed for a 25 mm field of view. They are made of special glass to enable maximum transmission. This makes them powerful photon collectors in fluorescence microscopy.
10X Leica PL Fluotar objective
NA: 0.32 / Free working distance: 11.13 mm
Ideal for whole organism, cell counting (proliferation assays), live/dead, and apoptosis applications.
PL Fluotars are powerful universal objectives with outstanding chromatic correction for at least three wavelengths which makes them suitable for fluorescence imaging.
Field planarity (PL) is computed for a 25 mm field of view. They are made of special glass to enable maximum transmission. This makes them powerful photon collectors in fluorescence microscopy.
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20X Leica PL Fluotar objective
NA: 0.40 / Free working distance: 7.45 mm
Ideal for cellular, neurons/neurite tracing, puncta counting, and cell scoring.
PL Fluotars are powerful universal objectives with outstanding chromatic correction for at least three wavelengths which makes them suitable for fluorescence imaging.
Field planarity (PL) is computed for a 25 mm field of view. They are made of special glass to enable maximum transmission. This makes them powerful photon collectors in fluorescence microscopy.
40X Leica PL Fluotar objective
NA: 0.60 / Free working distance: 3.0 m
Correction Collar
Ideal for organelles, puncta counting for small punctae (internalization, endocytosis, phagocytosis), and organelle studies for quantifying or analyzing organelle shape/morphology.
PL Fluotars are powerful universal objectives with outstanding chromatic correction for at least three wavelengths which makes them suitable for fluorescence imaging.
Field planarity (PL) is computed for a 25 mm field of view. They are made of special glass to enable maximum transmission. This makes them powerful photon collectors in fluorescence microscopy.
63X Leica PL Fluotar objective
NA: 0.70 / Free working distance: 2.0 mm
Correction Collar
Ideal for viral titer, small object imaging (i.e. yeast, phytoplankton, bacteria), and small molecule imaging (RNA in situ hybridization).
PL Fluotars are powerful universal objectives with outstanding chromatic correction for at least three wavelengths which makes them suitable for fluorescence imaging.
Field planarity (PL) is computed for a 25 mm field of view. They are made of special glass to enable maximum transmission. This makes them powerful photon collectors in fluorescence microscopy.
FPO
FPO
FPO
FPO
FPO
click on an objective below for additional information
ImageXpress Pico utilizes user-exchangeable filter cubes
click on a filter cube below for additional information
Cy5
TRITC
FITC
DAPI
Texas Red
White
Light
CFP
RGB
Cy5 Filter Cube
Excitation Range: 610 - 650 / Emission Range: 675 - 720
Example Fluophores (Ex/Em)
Example Targets
Live, Fixed, Both
Example Applications
"APC (allophycocyanin) (651/660),
Alexa Fluor™ 633 (631/650),
Alexa Fluor™ 647 (650/670),
Alexa Fluor™ 635 (634/647),
Alexa Fluor™ 660 (663/691),
Alexa Fluor™ 680 (679/704), DyLight 633 (638/658),
DyLight 649 (654/673),
Cy5 (648/666),
Cy5.5 (678/696)"
Conjugates
Fixed
Staining cells, organelles, and labeling proteins with conjugated peptides, antibodies, or streptavidin.
"DRAQ5 ™ (650/680), NuclearMask Deep Red (635/687), NuclearMask Red (622/644), NucRed™ Live 647 (638/686), SYTO 17 (619/638), SYTO 59 (621/644), SYTO 60 (649/681),
SYTO 62 (650/681), SYTO 63
(657⁄673)"
DNA/Nuclei
Both
Staining nuclei with cell-permeant DNA binding dyes
Close Table
TRITC Filter Cube
Excitation Range: 509-552 / Emission Range: 573-613
Example Fluophores (Ex/Em)
Example Targets
Live, Fixed, Both
Example Applications
CellTracker™ Orange (547/575), CellTracker™ Red (585/612), CellTracker™ Red CMTPX (577⁄602), CellTracker™ DiI (551/568)
Cell Tracking, Migration, and Proliferation
Live
Multigeneration studies of cell tracking, migration, and proliferation with cell-permeant dyes that become impermeant once inside the cell
"Alexa Fluor™ 546 (556/573), Alexa Fluor™ 555 (555/580), Cy3 (552/570), Cy3.5 (579/597), DyLight 594
(562/576), Tetramethylrhodamine (TRITC) (555/580)"
Conjugates
Fixed
Staining cells, organelles, and labeling proteins with conjugated peptides, antibodies, or streptavidin
BODIPY™ Red (587⁄615)
Lipids and Conjugates
Both
Fluorescent imaging of lipids, membrane, and other hydrophobic compounds. BIODIPY™ antibody and peptide conjugates for cellular
and protein labeling.
Close Table
NEXT PAGE
FITC Filter Cube
Excitation Range: 445-485 / Emission Range: 509-539
Example Fluophores (Ex/Em)
Example Targets
Live, Fixed, Both
Example Applications
BCECF (499/540)
Cell Viability / Intracellular pH
Live
Imaging live cells and monitoring intracellular pH in cell viability studies with a mixture of cell-permeable ester derivatives that yield BCECF upon hydrolysis by cytosolic esterases.
Calcein AM (494/517), CellTracker Green CMFDA (492/517)
Whole Cell Green (493/518)
Intracellular Esterases/Cell Viability, Tracking, Proliferation
Whole Cell
Live
Both
Utilizing intracellular esterases to generate a fluorescent, cell- permeable live cell stain for monitoring cell viability
Fixable, photostable whole cell stain
Close Table
DAPI Filter Cube
Excitation Range: 350-390 / Emission Range: 419-482
Example Fluophores (Ex/Em)
Example Targets
Live, Fixed, Both
Example Applications
CellTrace™ Calcein Blue (373/440), CellTrace™ Calcein Violet (401/451)
Intracellular esterases/Cell Viability, Proliferation, and Migration
Live
Cell viability and live cell analysis with cell-permeant short-term, live cell dyes that interact with intracellular esterases
ER-Tracker™ Blue-White DPX (371/554; long emission bandwith between 430 and 640 nm)
LysoSensor™ Blue (374/424) LysoTracker™ Blue (373/422)
Endoplasmic Recticulum
Lysosomes
Live
Live
Endoplasmic recticulum imaging with the cell- permeable ER-Tracker dye.
Fluorescent stains for imaging lysosomes and acid organelles
Close Table
Texas Red Filter Cube
Excitation Range: 350-390 / Emission Range: 419-482
Example Fluophores (Ex/Em)
Example Targets
Live, Fixed, Both
Example Applications
CellTrace™ Calcein Blue (373/440), CellTrace™ Calcein Violet (401/451)
Intracellular esterases/Cell Viability, Proliferation, and Migration
Live
Cell viability and live cell analysis with cell-permeant short-term, live cell dyes that interact with intracellular esterases
ER-Tracker™ Blue-White DPX (371/554; long emission bandwith between 430 and 640 nm)
LysoSensor™ Blue (374/424) LysoTracker™ Blue (373/422)
Endoplasmic Recticulum
Lysosomes
Live
Live
Endoplasmic recticulum imaging with the cell- permeable ER-Tracker dye.
Fluorescent stains for imaging lysosomes and acid organelles
Close Table
NEED TEXAS RED INFO
NEED TEXAS RED INFO
Close Table
NEED CFP INFO
CFP Filter Cube
Excitation Range: 350-390 / Emission Range: 419-482
Example Fluophores (Ex/Em)
Example Targets
Live, Fixed, Both
Example Applications
CellTrace™ Calcein Blue (373/440), CellTrace™ Calcein Violet (401/451)
Intracellular esterases/Cell Viability, Proliferation, and Migration
Live
Cell viability and live cell analysis with cell-permeant short-term, live cell dyes that interact with intracellular esterases
ER-Tracker™ Blue-White DPX (371/554; long emission bandwith between 430 and 640 nm)
LysoSensor™ Blue (374/424) LysoTracker™ Blue (373/422)
Endoplasmic Recticulum
Lysosomes
Live
Live
Endoplasmic recticulum imaging with the cell- permeable ER-Tracker dye.
Fluorescent stains for imaging lysosomes and acid organelles
NEED CFP INFO
Download dye and stain compatibility table
NEED CFP IMAGE
Click on a to the left for additional information
Return Home
Objectives
Filters
Labware
Interior
ImageXpress Pico utilizes easy-to-access,
user-exchangeable filter cubes and Leica objectives
click on a filter cube below for additional information
Flexibility to expand
click on a filter cube below for additional information
The ImageXpress Pico system accommodates various labware options including 6- to 384-well microplates, single and multiple slide holders, 35mm culture dish holders, and more.
Full Screen
Press ESC to exit full screen
ImageXpress Pico Filter Cubes have a magnetic pin that aligns the filter cube for easy exchange by the user.
Customizable to Meet Your Research Needs
The Molecular Devices ImageXpress Pico and S-LAB automated plate handler from PAA is optimized to work together, enabling a reliable solution for unattended sample analysis resulting in increased productivity, reduced cost, and consistent performance.
4 plate incubator shown.
Additional configuations available.
Robotic Automation
96-well Microplates
Accommodates a wide variety of labware including 4 slide holder, environmental control cassette, and 35mm culture dish holders.
6-well Cell Culture Plates
Single Slides
384-well Microplates
NEED NEW IMAGE
NEED NEW IMAGE
Download dye and stain compatibility table
Download dye and stain compatibility table
Download dye and stain compatibility table
Download dye and stain compatibility table
Download dye and stain compatibility table
CFP - 40X U2OS cells transduced with bacmam nucleus
Texas Red - MitoTracker Red stained BPAE cells (mitochondria)
DAPI - 20X U2OS cells with Hoechst stained nuclei
FITC - 40X BPAE cells stained with
AlexaFluor 488 phalloidin (actin stain)
Cy5 - 40X iPSC derived neurons stained for MAP2B zoomed in
Sale is subject to our Custom Product Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms. Highlighted features’ price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.
Choose from a list, run a search, or filter by specific criteria to find previous experiments to analyze.
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Home
Analyze your experiment results in a variety of view options to best suit your needs
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Quickly and easily switch between data visualization options - Thumbnail View
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Quickly and easily switch between data visualization options - Data View
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Quickly and easily switch between data visualization options - Heatmap
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Inspect individual wells
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Visualize your data by automatically generating scatter plots, bar graphs, tables, and even movies.
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Click to choose a protocol optimized to collect the most pertinent results and to ensue accurate segmentation.
Choose from a list of preconfigured protocols—from simple cell counting to sophisticated neurite tracing—optimized to collect the most crisp images, or customize your own to meet your specific research needs.
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Click to step through settings to customize your cell image acquisition to perfectly match your research needs
Easily step through settings to customize your cell image acquisition to perfectly match your research needs
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Easily change plate format or accept defaults.
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Adjust region selection settings quickly
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Run analysis on-the-fly by selecting from the list of predefined analysis modules without having to return to the main menu.
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Choose to analyze the entire acquisition area or select a specific region
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Select an entire plate or specific, individual wells
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Configure a time-lapse series, including discontinuous time-lapse, to generate movies and kinetic plots.
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Save your protocol settings for easy future access
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Run your protocol, and access from any network connected browser
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Click to return to main menu
Easily adjust image intensity settings, including activating Digital Confocal 2D on-the-fly image deconvolution
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Click to turn Digital Confocal ON/OFF
Digital Confocal On
Standard Widefield Image
Click on a to the left for additional information
4
3
2
1
Viral Infectivity
Custom Mimetas
2 Lane Protocol
Custom Mimetas
3 Lane Protocol
Pits and Vessicles
Protein Expression Index
Translocation
4
Multi-Color Cell Scoring
Neurite Tracing
Phagocytosis
Lysosomes
Mitochondria
Mitotic Index
3
Endocytosis
Internalization
Live Cells
Cell Scoring
Cell Scoring - Transmitted Light
Double Marker Expression
2
Angiogensis
Apoptosis
Autophagy
Cell Counting
Cell Count - Transmitted Light
Cell Differentiation
1
4
3
2
1
The ImageXpress Pico system accommodates various labware options including slide holders, 35mm culture dish holders, and more.
Significantly increase resolution and assay quality with Digital Confocal 2D on-the-fly deconvolution. The Digital Confocal Option restores light to its original point of origin, allowing you to decrease exposure time and improve statistical significance of your observations. Digital Confocal is seamlessly integrated into Pico’s fluorescent image acquisition workflow allowing you to capture fewer z-stacks, meaning faster scan speeds, with higher signal to noise data for more precise segmentation and analysis.
Simplify the identification of ROIs (regions of interest). Visualize your sample prior to acquisition using the virtual joystick to pan around the sample, and interactively adjust focus. Live Preview, featuring “click-to-center” functionality, continuously updates the image so you can easily navigate your sample to find the desired field of view. Whether you’re working on 96-well microplates, slides, or 35mm culture dishes, Live Preview helps you to quickly and easily focus in on what’s important to your research.
Live Preview Mode
Digital Confocal Option
Visualize your data by automatically generating scatter plots, bar graphs, tables, and even movies.
Visualize your data by automatically generating scatter plots, bar graphs, tables, and even movies.
View Rear Instrument Panel
View ImageXpress Pico Configurations
Full Screen
Press ESC to exit full screen
Return Home
Full Screen
Press ESC to exit full screen
Click on a to the left for additional information
View CellReporterXpress
ImageXpress Pico Standard Configuration
ImageXpress Pico with Environmental Control
Additional Options
ImageXpress Pico Standard Configuration
ImageXpress Pico with Environmental Control
OBJECTIVES:
Leica HC PL FLUOTAR 4X (NA 0.13) and 10X (NA 0.32), Transmitted light capability
FILTER CUBES:
FITC filter cube for fluorescence imaging
LIGHT SOURCE:
Solid state light source and optical train - a large imaging field of view is possible.
ENVIRONMENTAL CONTROL:
Capable of incubation up to 40°C, CO2 control between ambient and 15%, O2 control between 1 and 15% and ambient O2, and humidity control for minimizing evaporation.
LABWARE:
Microtiter plate adapter (6- to 384-wells) and 4-slide adapter
SOFTWARE:
CellReporterXpress Software license is included which enables 5 concurrent logins from a desktop or laptop with up to 2 logins from a tablet device per desktop login. License allows up to 10 computer cores to be run for distributed image analysis.
ANALYSIS:
Standard image analysis algorithms include cell counting, cell scoring, apoptosis, and other analysis modules for a total of 17 analysis modules.
SERVICE:
System includes 1 year MDCare PhD Technical Support Plan, and 1-year warranty covering parts & labor.
Click on a to the left for additional information
OBJECTIVES:
Leica HC PL FLUOTAR 4X (NA 0.13) and 10X (NA 0.32), Transmitted light capability
FILTER CUBES:
FITC filter cube for fluorescence imaging
LIGHT SOURCE:
Solid state light source and optical train - a large imaging field of view is possible.
LABWARE:
Microtiter plate adapter (6- to 384-wells) and 4-slide adapter
SOFTWARE:
CellReporterXpress Software license is included which enables 5 concurrent logins from a desktop or laptop with up to 2 logins from a tablet device per desktop login. License allows up to 10 computer cores to be run for distributed image analysis.
ANALYSIS:
Standard image analysis algorithms include cell counting, cell scoring, apoptosis, and other analysis modules for a total of 17 analysis modules.
SERVICE:
System includes 1 year MDCare PhD Technical Support Plan, and 1-year warranty covering parts & labor.
Additional Options
OBJECTIVES:
Leica HC PL FLUOTAR 20X (NA 0.40), 40X (NA 0.60), 63X (NA 0.70)
FILTER CUBES:
CFP, DAPI, Texas Red, TRITC, Cy5
CELLREPORTERXPRESS ANALYSIS:
Advanced Analysis Module
Z-Stacking*
Multi-wavelength Cell Scoring
METAXPRESS ANALYSIS:
MX Basic Software Bundle
MDCStore Xchange Service
Translocation
Neurite Outgrowth
Angiogenesis
Count Nuclei
Granularity
Transfluor
Live/Dead
Cell Health
Translocation Analysis Algorith**
Neurite Tracing Algorithm**
Granularity Analysis Algorithm**
Cell Scoring
Multiwavelength Cell Scoring
Monopole Detection
Cell Cycle
Nuclear Translocation HT
Cell Proliferation HT
Transfluor HT
Multiwavelength Translocation
Micronuclei
Mitotic Index
* included with Environmental Control
** included with Advanced Analysis Module
