Comparison of the RIMS-seq
and DNA-seq workflow
Confocal microscopic images of colon carcinoma cell line (HCT116) (DAPI + Texas Red-dNTP)
RADAR-seq: Genome-wide DNA damage detection
Retroviral vs Group II Intron-encoded Reverse Transcriptases – Similarities, Differences & Applications
Induro™ Reverse Transcriptase
Induro Reverse Transcriptase is a group II intron-encoded RT that exhibits high processivity, increased thermostability, and increased tolerance of inhibitors for the synthesis of cDNA from RNA. It is an ideal enzyme for challenging cDNA synthesis from long transcripts (>8 kb), RNAs with strong secondary structures, and RNA samples with inhibitors.
Advances in next generation sequencing: How researchers at NEB are working to improve our understanding of the genome, epigenome and transcriptome
Conducting innovative basic research has always been integral to
New England Biolabs’ philosophy; our scientists have contributed to the advancement of science and modern molecular biology technologies for almost 50 years.
In this article, find out how NEB scientists are applying their expertise in enzymology and NGS to address unmet needs in the analysis of genome-wide methylation, transcriptional start sites, full-length transcriptomes, chromatin accessibility, and much more.
NEB scientists have authored or co-authored over
1,500 publications.
EXPRESSIONS
A scientific update
A new class of performance
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The most common and well-characterized RTs are derived from retroviruses. Many commercially-available retroviral RTs have been genetically engineered with mutations that confer specific attributes, such as reduced RNase H activity, increased half-life and increased thermostability. A second family of RTs, distinct in sequence and domain organization, is found within the intron-encoded proteins of group II introns.
We are dedicated to inspiring young scientists and improving the quality of science education for students by providing reagents, educational materials and employee knowledge and time.
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Reverse Transcriptases (RTs)
NEB has an extensive portfolio of RTs, which are available as standalone products or have been incorporated into convenient master mixes or kits. For more details and access to the full product listing, visit www.neb.com/rt.
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NEB
Issue III 2022
NEB's Course Support Program
In this Q&A with Penny Devoe, an Associate Director in the Marketing Department at NEB, Penny shares insight on NEB’s efforts to support educational outreach through our course support program.
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NGS library prep?
We’ve got you covered.
For 13 years, NEB® has addressed library prep challenges by offering solutions that streamline workflows, minimize inputs and improve library yield and quality. In fact, use of NEBNext® reagents has been cited in >20,000 publications.
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NEBNext® Ultra™ II DNA Sample Prep Solutions – now featuring PCR-free workflows
Applications
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While retroviral RTs are widely available and work well in many well-established protocols, intron-encoded RTs offer some advantages over retroviral RTs, and can be a better choice for cDNA synthesis from long transcripts, RNAs with strong secondary structures, and RNA samples with inhibitors.
from New England Biolabs®
Retroviral
Group II intron
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Right Whales and Lobster boats:
Saving icons of New England
Stretching up to 52 feet long and weighing up to 62 tons, the North Atlantic right whale is one of the world’s largest animals—and one of the most endangered whales.
Scientists estimate that there are fewer than 350 right whales remaining.
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Long cDNA synthesis
(up to 20 kb)
Impure RNA samples
