Clone with Confidence.
Whether you are performing your first cloning experiment or constructing multi-fragment DNA assemblies, NEB has the solution for you. Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder® HiFi DNA Assembly and NEBridge® Golden Gate Assembly. We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. When you are looking to clone with confidence, think of NEB.
Enhancing Molecular Diagnostics in Regulated Markets with Lyophilized Assays
Molecular diagnostic assays are developed to identify and quantitate specific DNA, RNA, or protein biomarkers found within samples of interest. In many regions, molecular diagnostic testing may only be performed by procuring assays subject to regulatory approval, or with validated tests and workflows in approved laboratories.
Historically, molecular diagnostic assays have utilized cold storage reagents, which require controlled temperature cabinets and dry or wet ice shipment. However, recently, there has been a dramatic increase in the need for molecular diagnostic testing in near-patient and point-of-care settings, and ambient-stored reagents are a key factor in enabling this testing. This requirement has driven the markets towards lyophilized reagents that can be stored and shipped at ambient temperatures.
EXPRESSIONS
A scientific update
Amplification-based Molecular Diagnostics
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People are moving pretty quickly in different areas of sequencing. So we have a bunch of computational folks working closely with the lab folks.
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High-throughput cloning is a molecular biology method for assembling large numbers of DNA sequences, suchas genes, open reading frames (ORFs) or highly repetitive gRNAs, to create libraries and enable screening of constructs, protein expression or protein function.
With the integration of automation, researchers can scale up and increase throughput to hundreds or thousands of reactions, save time and money with rapid workflows and miniaturized volumes, and improve reproducibility with automated complex mixing that reduces manual errors.
NEB offers a wide variety of products that enable highly efficient and accurate DNA assembly and mutagenesis, sequencing, cell-free protein synthesis and purification that are all amenable to high-throughput workflows and automation devices. To learn more, visit www.neb.com/AutomateWithConfidence.
NEB
Issue I 2023
How Bioinformatics Informs Sequencing at NEB
In a recent Lessons from Lab & Life™ podcast, Lydia Morrison chatted with NEB Development Group Leader Brad Langhorst about how bioinformatics informs sequencing, from primer design to analysis. Access the Lessons from Lab & Life podcast at www.neb.com/podcasts to hear the entire interview and to learn more about how the bioinformatics team at NEB support R&D.
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Read Article
Read Article
View PDF
View PDF
FEATURED PRODUCTS FOR
HIGH-THROUGHPUT DNA ASSEMBLY
• Perform less sequencing and screening of constructs with high-fidelity, virtually
error-free assembly
• Enjoy compatibility with synthetic dsDNA fragments, such as gBlocks™, and
ssDNA oligos
• Save time by avoiding PCR clean-up, simplifying your workflow
• Supports miniaturization with nanoliter scale volumes
• Easily adaptable to multiple site-directed mutagenesis
• Design primers and assemblies quickly and easily with our free online tool,
NEBuilder Assembly Tool
Learn More
Learn More
View Product Listing
View Product Listing
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Genes in Space™ is a unique opportunity for young scientists to launch their ideas into orbit
Genes in Space is a free competition, founded and run by
miniPCR bio™ (www.minipcr.com) and Boeing®, for 7th- to 12th-grade students, challenging them to come up with experiments evaluating how living things react to space conditions. Every year, about 200 schools participate in the contest, but students can enter independently with sponsorship from an adult (a teacher or parent).
My favorite part is working with the students and seeing all their excitement and unique ideas. One of the things that I loved about Pristine’s experiment from the beginning was how creative she was. Her ability to tie different aspects of different fields of biology all together in this experiment was very impressive.
”
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Explore the wise choice at
CloneWithNEB.com
Automate with Confidence -
High-throughput Solutions for Cloning and Automation
NEBuilder® HiFi DNA Assembly
Ordering Information
PRODUCT NEB # SIZE
NEBuilder HiFi DNA
Assembly Master Mix
E2621S/L/X
10/50/250 reactions
NEBuilder HiFi DNA
Assembly Cloning Kit
NEBuilder HiFi DNA
Assembly Bundle for Large Fragments
E5520S
E2623S
10 reactions
20 reactions
NEBridge® Golden Gate Assembly
• Experience high efficiency within regions of high GC content and areas of repeats
• Supports miniaturization
• Enjoy compatibility with synthetic dsDNA fragments, such as gBlocks™
• Find flexibility with NEBridge Ligase Master Mix (NEB #M1100) and your choice
of Type IIS restriction enzymes
• Use our free online tools to design complex, high-fidelity Golden Gate Assemblies
– NEBridge Golden Gate Assembly Tool – design primers, predict overhang
fidelity and find optimal junctions
– NEBridge Ligase Fidelity Tools – visualize overhang ligation preferences,
predict high fidelity junction sets and split DNA sequences for scarless
high-fidelity assembly
Ordering Information
NEBridge Golden Gate Assembly Kit (BsmBI-v2)
NEBridge Golden Gate Assembly Kit
(BsaI-HF® v2)
NEBridge Ligase
Master Mix
E1602S/L
E1601S/L
M1100S
20/100 reactions
20/100 reactions
50 reactions
PRODUCT NEB # SIZE
FEATURED PRODUCTS FOR
HIGH-THROUGHPUT MUTAGENESIS
• Quickly create mutant libraries using primers designed with our free online
tool, NEBaseChanger®
• Scale-up, or miniaturize with individual master mix formats compatible with
automation
• Use Q5® Hot Start High-Fidelity 2X Master Mix (NEB #M0494) for PCR
amplification
• Use KLD Enzyme Mix (NEB #M0554) for kinase, ligase and DpnI
enzymaticactivities in single mix
• Reduce screening of correct mutants with extremely accurate and robust
Q5® High-Fidelity DNA Polymerase
• Take advantage of automation compatible room temperature reaction set up
using a hot start polymerase
Ordering Information
Q5® Hot Start
High-Fidelity
2X Master Mix
KLD Enzyme Mix
M0494S/L/X
M0554S
100/500/500 reactions
25 reactions
PRODUCT NEB # SIZE
• Using NEBuilder HiFi DNA Assembly, perform multi-site mutagenesis or
combinatorial mutagenesis for diverse multi-site mutant library creation
and screening.
Ordering Information
NEBuilder HiFi DNA Assembly Master Mix
E2621S/L/X
10/20/250 reactions
PRODUCT NEB # SIZE
Single site-directed mutagenesis
Multi site-directed mutagenesis
Our extensive expertise in amplification, including PCR, qPCR, RT-qPCR and isothermal amplification has allowed us to develop optimized enzymes for a variety of applications, including incorporation into diagnostics.
Updated NEB Web Tools
Achieve effortless DNA restriction digests and site-directed mutagenesis reactions with our updated web tools, designed to ensure trouble-free experimentation.
NEBaseChanger helps researchers design site-directed mutagenesis experiments using NEB’s Q5 Site-Directed Mutagenesis Kit.
• Design primers for use in site-directed mutagenesis reactions
• Calculate a custom annealing temperature taking into account mismatches
• Download a summary of primer information, sequence of final construct
and recommendation protocol
We have recently enhanced the capabilities of NEBaseChanger. New features include the following:
NEBcutter was originally designed to assist users with restriction digests and traditional molecular cloning workflows, and quickly became one of our most popular online tools.
• Find restriction enzyme sites within a DNA sequence
• Visualize a virtual restriction digest on a gel to compare against results at the bench
• Determine whether DNA methylation will impact your restriction digest
Additionally, NEBcutter V3.0 offers an improved user interface and includes the following features::
Generate multiple primers using ambiguous bases
Opt for mutation sequences in
primer tails
Substitutions based on nucleotide or amino acid position
Batch input to simultaneously generate multiple primer sets
Download results
as a PDF
Filter by types of REs (i.e., blunt, 8-base cutter, etc)
Add multiple enzymes at one time
Log in to save your projects and view from various browsers
Visualize digestion
on a virtual gel
Quickly find open reading frames with our enhanced ORF Finder
TOP ROW: Jake Miller, Timur Shtatland, Ariel Erijman, Brad Langhorst
BOTTOM ROW: Jian Sun, Laura Blum,Caiden Kumar, Gautam Naishadham
NOT PICTURED: Matt Angel, Matt Campbell
phi29-XT RCA Kit
Featuring phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability, sensitivity, and capable of generating high product yield in a short reaction time, the phi29-XT RCA Kit includes everything needed for rolling circle amplification (RCA).
The phi29-XT RCA Kit (NEB #E1603) is a fast, simple to use and highly versatile kit containing all the required components for rolling circle amplification (RCA) using a random primer mix. The kit delivers high yields of DNA products from a variety of starting materials including purified circular DNA or bacterial cells. This kit is ideal for various DNA applications such as DNA sequencing, cell-free DNA enrichment, cell-free protein expression and DNA biosensors.
Advantages
• Amplify from as little as 1 fg of circular DNA input
• Flexible protocol offers compatibility with different types of input material
• Enables multiple downstream applications without further processing steps
• Kit includes dNTPs and exo-resistant random primers
Pristine Onuoha joined the Genes in Space team in Boston at a Space Biology Camp event.
Ana Karla Cepeda Diaz, Harvard Grad Student & Pristine Onuoha’s Genes in Space mentor
NEBuilder® HiFi DNA Assembly
Single site-directed mutagenesis
Multi site-directed mutagenesis
Read Article
Read Article
Learn More
Learn More
CloneWithNEB.com
Listen to Podcast
Listen to Podcast
Try Tool
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NEBridge® Golden Gate Assembly
FEATURED PRODUCTS FOR
HIGH-THROUGHPUT DNA ASSEMBLY
FEATURED PRODUCTS FOR
HIGH-THROUGHPUT MUTAGENESIS
FEATURED PRODUCTS FOR
HIGH-THROUGHPUT DNA ASSEMBLY
FEATURED PRODUCTS FOR
HIGH-THROUGHPUT MUTAGENESIS
FEATURED PRODUCTS FOR
HIGH-THROUGHPUT MUTAGENESIS
FEATURED PRODUCTS FOR
HIGH-THROUGHPUT DNA ASSEMBLY