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NEB's CLIA-certified onsite testing program utilizes saliva, which is collected using a micropipette tip and microfuge tube, and loop-mediated isothermal amplification (LAMP).
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NEB has long been a leader in enzymes for molecular diagnostics, with isothermal amplification and LAMP of particular focus.
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The sample position on the plate is photographed and recorded using the QR code.
Introducing LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG
LyoPrime Luna offers a robust, versatile RT-qPCR option in a shelf-stable lyophilized format
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NEBNext® ARTIC SARS-CoV-2
Library Prep Kit
(Illumina®)
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Mitigating Risk and Ensuring Consistent Supply Chain Through Internal SARS-CoV-2 Testing with RT-LAMP
The COVID-19 pandemic posed a significant threat to the functioning of laboratories and workplaces worldwide, including here at New England Biolabs® (NEB®) and our biotechnology operations in Massachusetts. As the pandemic disruptions began to take hold, it was essential to maintain our ability to produce critical materials for molecular diagnostic tests and mRNA vaccine development.
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We established a CLIA-certified onsite testing program utilizing saliva and colorimetric LAMP, and to date we have processed over 35,000 SARS-CoV-2
tests for our employees.
EXPRESSIONS
A scientific update
SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit
The SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit utilizes loop-mediated isothermal amplification for use in the analysis of SARS-CoV-2, the novel coronavirus that causes COVID-19.
NEW PRODUCT
It's prime time!
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Supplied as a lyophilized cake, the LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG enables sensitive detection of target RNA sequences in a room temperature-stable format. This product contains the same versatile features and strong performance as the liquid version: Luna® Probe One-StepRT-qPCR 4X Mix with UDG (NEB #M3019).
NEBNext® ARTIC SARS-CoV-2
FS Library Prep Kit
(Illumina®)
Learn more
NEBNext® ARTIC SARS-CoV-2 Companion Kit
(Oxford Nanopore Technologies®)
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Benefit from almost 50 years of experience in molecular biology & enzymology
Partner with our OEM & Customized Solutions team to find the best solution for your needs
Take advantage of our expanded manufacturing capabilities
Bringing more COVID-19 testing to Africa
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With so few Africans vaccinated against COVID-19, simple and sensitive tests are critical for saving lives. NEB is donating reagents to aid in the development of diagnostic tests (cLAMP) that can be used instead of PCR and can deliver rapid results detected by a simple color change. They are ideally suited for resource-poor areas by not requiring expensive laboratory equipment.
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It's time to think differently.
Find out how NEB can support your infectious disease research and development.
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NEB
Issue I 2022
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Strong linearity and reproducibility over a broad dynamic range (8-log, 1 µg to
0.1 pg human total RNA)
A. RT-qPCR targeting human -actin was performed using either the LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG (NEB #L4001) or Luna Probe One-Step 4X Mix with UDG (NEB #M3019) over an 8-log range of input template concentrations (1 μg – 0.1 pg Jurkat total RNA) with 4 replicates at each concentration, run on an ABI QuantStudio® 6 Flex real-time instrument. Reactions (20 μl) included primers at 400 nM each and and probe at 200 nM, and followed the product recommended cycling conditions.
B. Standard Curve results were substantially equivalent for the lyophilized (gold) and liquid-format (blue) mixes, with strong linearity and reproducibility observed, even at the lowest concentrations tested.
β
NEBNext ARTIC Kits for SARS-CoV-2 sequencing
The NEBNext ARTIC kits meet the need for reliable, accurate and fast methods for sequencing SARS-CoV-2 variants, with Oxford Nanopore Technologies® or Illumina® sequencing.
Updated NEBcutter V3.0
NEBcutter was originally designed to assist users with restriction digests and traditional molecular cloning workflows, and quickly became one of the most popular online tools available at neb.com. NEBcutter is used to:
• Find restriction enzyme sites within a DNA sequence
• Visualize a virtual restriction digest on a gel to compare against
results at the bench
• Determine whether DNA methylation will impact your restriction digest.
Try NEBcutter
Try NEBcutter
Bringing the International Space Station into the molecular age
In recent years, Dr. Wallace and her team have been moving microbial identification on the ISS into the molecular age utilizing techniques such as PCR and nucleic acid sequencing.
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Christina Koch demonstrates pipetting onboard the ISS as she extracts and purifies DNA for downstream nanopore sequencing.
Monarch DNA & RNA Purification Kits – Designed with sustainability in mind
®
®
View Additional Details
View Additional Details
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25%
25%
Access product formats, such as GMP-grade*, lyophilized,
lyo-ready and glycerol-free
Be confident in your product performance with our expanded quality and regulatory systems
Download results as a PDF
Filter by types of REs (i.e., blunt,
8-base cutter, etc)
Add multiple enzymes at one time
Log in to save your projects and view from various browsers
Visualize digestion on a virtual gel
Quickly find open reading frames with our enhanced ORF Finder
Reduced lab waste
Significantly less plastic as compared to leading supplier
Monarch kits still deliver high yields, purity and performance
Thinner-walled columns
Reduction in total plastic without affecting performance
Buffer bottles
Carefully designed to minimize plastic usage
Flexible purchasing options
Buffers and columns sold separately
Purchase only what you need and avoid wasted materials
Same performance, design and formulations
Standalone products are the same components that are included in complete kits
No excessive packaging
Sturdy, reusable boxes at just the right size
Carefully designed to eliminate empty space, versatile Monarch boxes can be reused anywhere
Concise protocol cards replace printed manuals
Both cards and manuals are available online as PDFs
Sustainable & recyclable packaging
Sourced for recyclability
All components are purposefully sourced for recyclability
Recycled paper
Used to make the kit boxes, inserts and paper materials
Instructions for recycling kit components
Can be found on product packing or online
Eco-friendly printing
Printing of boxes and packaging powered by green sustainable sources such as wind
NEBNEXT ARTIC KIT OPTIONS
cDNA synthesis
MODULE
ARTIC SARS-CoV-2
RT-PCR Module
NEB #E7626
Targeted amplification
Ultra II FS
~150 bp library
PE: 2 X 75 bp
NEB #E7658
Ultra II
~400 bp library
PE: 2 X 250 bp
NEB #E7650
ILLUMINA
SEQUENCING
ONT Companion Kit
~400 bp library
2 – 24 hr run
NEB #E7660
OXFORD NANOPORE SEQUENCING
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Dr. Peggy Whiston sets up her laboratory bench onboard the ISS as part of the Genes in Space-3 experiment. During this investigation, Dr. Whitson demonstrated the first identifications of microbes collected and cultured off Earth.
Ricky Arnold was the first astronaut onboard the ISS to run the swab-to-sequencer methodology and successfully sequence native human RNA.
®
Pre-hydration
(before)
Hydration
Post-hydration
(20 sec. after)
Strong linearity and reproducibility over a broad dynamic range (8-log, 1 µg to
0.1 pg human total RNA)
Standard Curve results were substantially equivalent for the lyophilized (gold) and liquid-format (blue) mixes
At NEB, we continuosly strive to promote ecologically sound practices and environmental sustainability in order to protect our natural resources, both locally and globally. For over five years, we have been designing our Monarch DNA and RNA purification kits & products with sustainability in mind by purposefully reducing plastics and packaging without sacrificing performance and quality.
Click the icons below to learn more.
Strong linearity and reproducibility over a broad dynamic range (8-log, 1 µg to
0.1 pg human total RNA)
Learn More
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* “GMP-grade” is a branding term NEB uses to describe reagents manufactured at our Rowley, MA facility, where we utilize procedures and process controls tomanufacture reagents under more rigorous conditions to achieve more stringent product specifications, and in compliance with ISO 9001 and ISO 13485 qualitymanagement system standards. NEB does not manufacture or sell products known as Active Pharmaceutical Ingredients (APIs), nor do we manufacture products incompliance with all of the Current Good Manufacturing Practice regulations.
NEBNext® ARTIC SARS-CoV-2
Library Prep Kit
(Illumina®)
Learn More
Learn More
NEBNext® ARTIC SARS-CoV-2
FS Library Prep Kit
(Illumina®)
Learn more
Learn more
NEBNext® ARTIC SARS-CoV-2 Companion Kit
(Oxford Nanopore Technologies®)
Learn More
Learn More
View PDF
View PDF
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