Welcome to
Monarch
®
Your solution for nucleic acid purification
Purification of DNA and RNA is an essential step in many molecular biology workflows, including enzyme digests, transformation, electrophoresis, RT-PCR, RT-qPCR and library preparation for next gen sequencing. Monarch kits enable quick and easy purification of high-quality DNA and RNA, suitable for use in a variety of downstream applications. Recover pure, intact DNA and RNA in minutes with fast, user-friendly protocols and optimized buffer systems, and focus your time on the experiments that will drive your research forward.
What are you working with?
Plasmid Purification
(Miniprep)
DNA Cleanup and
Gel Extraction
Genomic DNA
Purification
Plasmid Purification (Miniprep)
New version of plasmid miniprep kit, featuring upgraded spin columns
Elute in as little as 30 µl for highly concentrated DNA, with yields up to 20 µg
Easy to follow workflow with colored buffer system
Available Products for Plasmid Purification (Miniprep):
Monarch Spin Plasmid Miniprep Kit
(columns also sold separately)
Quality and quantity was OUTSTANDING!
Researcher,
Case Western Reserve University
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Less buffer retention as advertised! The indicator system prevents under and over-mixing for lysis and neutralization step, minimizing genomic DNA fragmentation.
Researcher,
University of Massachusetts, Amherst
I like the clear instructions and streamlined, small footprint packaging.
Researcher, Pacific Northwest Diabetes Research Institute
It was great for teaching.
My students knew when the solutions were mixed properly. They liked the color changes.
Professor, Ripon College
You don't have to wash multiple times with each wash buffer. Also, DNA recovery was very high and pure.
Researcher, Walsh University
Simple to use, good quantity and quality of purified DNA.
Principal Investigator, London, UK
We have been using Qiagen for a very long time but when compared to the Monarch kit, we were surprised at how much more product we were able to collect. Great kits, easy to use protocols!! A+
Researcher, Yale University
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It is simply perfect!
Researcher,
University of Massachusetts, Amherst
I loved the Monarch Kit. It is way better than the Qiagen due to the position and size of the filter in the columns.
Researcher,
University of California, San Francisco
In comparison, the yield of DNA I'm getting from the Monarch kit is 5 to 10 times greater than with the Qiagen. Also, the quality of DNA is also better (based on Nanodrop absorbance curve). Finally, the Monarch kit is easier to use.
Researcher, Yale University
The Monarch kit was easy to use, the clever column design prevented EtOH carryover and allowed elution in a smaller volume. The increased DNA concentration is a real benefit for the multi-fragment ligations our lab routinely performs. Overall a great kit at a competitive price.
Research Scientist, OR Health and Science University
In our lab we routinely use gel purification kits. A major problem is inconsistency of sample recovery and less yield. I recently tried Monarch purification kit along with Promega kit on the same PCR samples using half of PCR reaction in each. And that was the "AHA moment" for me, With Monarch I got 65 ng/μl in total 25 μl of elution volume whereas Promega gave me 18 ng/μl in same volume. Totally satisfied, hope to get same consistent yield every time.
Research Assistant, ND State University
I have been struggling to isolate enough product from the MinElute PCR cleanup and gel extraction kit for my PCR cloning project to be successful. We tried the Monarch PCR cleanup kit as a last-ditch effort and our ligation reaction was successful on the first try!
Assistant Specialist, University of California Irvine
Elutions in small volume worked great, and allowed us to clone the fragment with no additional manipulations!
Assistant Professor, Haverford College
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DNA Cleanup and Gel Extraction
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Available Products for DNA Cleanup and Gel Extraction:
Monarch Spin PCR & DNA Cleanup Kit (5 μg)
Monarch Spin DNA Gel Extraction Kit
Genomic DNA Purification
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Available Products for Genomic DNA Extraction and Purification:
Monarch Genomic DNA Purification Kit (columns also sold separately)
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Purify high quality genomic DNA (gDNA) from a broad range
of sample types (cells, blood, tissues, and more)
Achieve excellent yields with extremely low (typically <1%) residual RNA contamination (RNase A is included)
Isolate high molecular weight gDNA (peak size typically
≥50 kb)
Take advantage of user-friendly protocols with fast and efficient lysis steps
Quickly purify DNA after enzymatic reactions and from agarose gels
Simple PCR cleanup in only minutes
Elute in as low as 5 µl for highly concentrated DNA
Prevent buffer retention/salt carry over with unique, optimized column design
Purify oligos/ssDNA/small DNA fragments with a simple protocol modification
Significantly less plastic by design in columns and kit compared
to leading suppliers
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I can elute my DNA in a volume as small as 6 μl. I didn't have a lot of starting material to begin with so this helped concentrate my DNA too. I also ran a sample of my DNA after the clean-up for good measure and it was very clean!
Researcher,University of Massachusetts Medical School
I often purify small amounts of inserts for ligations, so I appreciate the ability to elute in small volumes. I also very much appreciate the reduction of paper and plastic waste.
Research Associate, Indiana University
Better yield and quality; easy protocol and good and consistent results among samples.
Gisele,Research Associate,
Mississippi State University
I've been using the RNeasy kit for some time, but I've been unable to have much luck. It seems geared toward eukaryotes and I've been working with Gram Positive prokaryotes… I tried the NEB [Total] RNA Miniprep Kit and it was much more straightforward. I also got excellent results the first time I used it.
Kyle, Postdoc Researcher, University of CA, Berkeley
I repeated the RNA extraction using [NEB’s Monarch Total RNA Miniprep Kit]. The quality of RNAs were checked using Tape Station. And all four samples had RIN between 8.3 to 9.3, so they are all good…Your RNA extraction kit is easy to use and saves time.
Researcher, UCL Great Ormond Street Institute of Child Health
I compared the yield of RNA extracted and the purity of the RNA based on spectrometer (Nanodrop). When I compared to my usual RNeasy kit from Qiagen to the Monarch sample, I obtain 5 times more RNA from my sample as well as higher purity of my sample with the Monarch RNA mini-prep.
Researcher, Iowa State University
I liked the consistency, ease of use, and the fact that everything you need (e.g. DNase, Proteinase K) comes in the kit. Both the concentration and quality of the isolated RNA from the Monarch Kit were slightly better than the GeneJet kit. The bigger difference was in the consistency of the RNA isolations between samples--it was much better with the Monarch Kit.
Natalie, PhD. Student, Winnipeg, CA
I’ve had a go extracting RNA from a couple of different samples using the Monarch kit, in parallel with Trizol extractions from duplicate samples. The initial results, nanodrop analysis of the extracted RNA, were very positive.
Jessica Purcell, Pirbright Institute, United Kingdom
Total RNA Extraction and Purification
Purify total RNA from multiple sample types (blood, cells, tissues and more) with a single kit
Includes DNase I and gDNA Removal Columns for efficient
DNA removal
Effectively purify RNA of all sizes, including small RNA (<200 nt)
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Available Products for Total RNA Extraction and Purification:
Monarch Total RNA Miniprep Kit
(columns also sold separately)
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Easy to use and fast purification. Exponentially better than precipitation methods with great recovery.
Researcher,
Oregon Health and Sciences University
RNA Cleanup
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Purify RNA from various reactions including in vitro transcription (IVT), DNase I treatment, labeling and capping
Clean up and concentrate RNA after enzymatic reactions and after extraction protocols, including TRIzol®
Obtain clean RNA in minutes with fast and simple protocols
Select from 3 binding capacities: 10 µg, 50 µg and 500 µg
Available Products for RNA Cleanup:
Monarch RNA Cleanup Kit (10 µg) (columns also sold separately)
Monarch RNA Cleanup Kit (50 µg) (columns also sold separately)
Monarch RNA Cleanup Kit (500 µg) (columns also sold separately)
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Total RNA Extraction
and Purification
RNA Cleanup
Sample type: Yeast (fresh mycelium)
Compared to: Qiagen RNeasy Mini Kit
Sample type: Gram Positive Bacteria
Compared to: Qiagen RNeasy Mini
Sample type: CD34 cells
Sample type: Oat Protoplast Cells
Compared to: Qiagen RNeasy Plant Mini Kit
Sample type: Mouse Heart
Compared to: Thermo Fisher Scientific GeneJet RNA Isolation Kit
Sample type: RNA from Virus in cell culture supernatant Compared to: TRIzol extraction
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As with other Monarch kits, the design of the filter (no bezel) prevents ethanol retention, which can be an annoying problem with other kits. We've switched to NEB Monarch kits for all of our DNA purifications.
Researcher,
OR Health and Sciences University
Sample Type: Leishmania donovani parasites
This kit yielded the highest purity DNA I have ever seen from a commercial spin column kit.
Researcher, Lake Superior State University
Sample type: Tissue
It was easy to use and produce good quality, long DNA.
Researcher, University of Strathclyde
Sample type: Bacteria
It works amazingly well for blood samples. I found the kit to be very easy and very quick to use. Probably the easiest protocol yet. The ability of this kit to do a single lysis step is great. It took me less than 20 minutes to go from sample to great quality DNA out of this prep.
Staff Scientist, Minnesota
Sample type: Blood
Fast and easy to use, can get both genome DNA and viral DNA from virus infected cells.
Researcher, Yale University
Sample type: Cultured cells
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Products
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HMW DNA
Extraction
We’ve had great success with obtaining HMW DNA for long-read sequencing from a variety of cell types, using less input and obtaining a comparable yield…It is straightforward and easy to use.
Inswasti Cahyani & Matt Loose, DeepSeq, University of Nottingham
Excellent fragment length as per TapeStation, excellent sequencing
on Oxford Nanopore Technologies MinION. This solved our bottleneck.
Dr. Scott Lindner,
Pennsylvania State University
Based on my extensive experience with gDNA extractions and manipulations, I think NEB has developed an industry leading kit in the Monarch T3060.
Dr. Joel Berry,
Felix Biotechnology
The NEB HMW Kit blows the Qiagen [QIAamp Blood] kit out of the water by all metrics.
Dr. Scott Lindner,
Pennsylvania State University
High Molecular Weight DNA (HMW DNA) Extraction
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Quickly and easily isolate HMW DNA from cells, blood, tissues, bacteria, and other sample types
Achieve DNA into the Megabase (Mb) size range
Tune the size of isolated DNA by adjusting the agitation speed during lysis
Obtain best-in-class yields of highly pure and intact DNA, with efficient RNA removal
Experience excellent performance in long read sequencing
Available products for HMW DNA Extraction:
Monarch HMW DNA Extraction Kit for Cells & Blood
Monarch HMW DNA Extraction Kit for Tissue
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Genomic DNA
Purification
As with other Monarch kits, the design of the filter (no bezel) prevents ethanol retention, which can be an annoying problem with other kits. We've switched to NEB Monarch kits for all of our DNA purifications.
Researcher, OR Health and Sciences University
Sample Type: Leishmania donovani parasites
This kit yielded the highest purity DNA I have ever seen from a commercial spin column kit.
Researcher, Lake Superior State University
Sample type: Tissue
It was easy to use and produce good quality, long DNA.
Researcher, University of Strathclyde
Sample type: Bacteria
It works amazingly well for blood samples. I found the kit to be very easy and very quick to use. Probably the easiest protocol yet. The ability of this kit to do a single lysis step is great. It took me less than 20 minutes to go from sample to great quality DNA out of this prep.
Staff Scientist, Minnesota
Sample type: Blood
High Molecular Weight DNA (HMW DNA) Extraction
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Quickly and easily isolate HMW DNA from cells, blood, tissues, bacteria, and other sample types
Achieve DNA into the Megabase (Mb) size range
Tune the size of isolated DNA by adjusting the agitation speed during lysis
Obtain best-in-class yields of highly pure and intact DNA, with efficient RNA removal
Experience excellent performance in long read sequencing
Available products for HMW DNA Extraction:
Monarch HMW DNA Extraction Kit for Cells & Blood
Monarch HMW DNA Extraction Kit for Tissue
Learn More
Learn More
Genomic DNA
Purification
As with other Monarch kits, the design of the filter (no bezel) prevents ethanol retention, which can be an annoying problem with other kits. We've switched to NEB Monarch kits for all of our DNA purifications.
Researcher, OR Health and Sciences University
Sample Type: Leishmania donovani parasites
This kit yielded the highest purity DNA I have ever seen from a commercial spin column kit.
Researcher, Lake Superior State University
Sample type: Tissue
It was easy to use and produce good quality, long DNA.
Researcher, University of Strathclyde
Sample type: Bacteria
It works amazingly well for blood samples. I found the kit to be very easy and very quick to use. Probably the easiest protocol yet. The ability of this kit to do a single lysis step is great. It took me less than 20 minutes to go from sample to great quality DNA out of this prep.
Staff Scientist, Minnesota
Sample type: Blood
Fast and easy to use, can get both genome DNA and viral DNA from virus infected cells.
Researcher, Yale University
Sample type: Cultured cells
High Molecular Weight DNA (HMW DNA) Extraction
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Quickly and easily isolate HMW DNA from cells, blood, tissues, bacteria, and other sample types
Achieve DNA into the Megabase (Mb) size range
Tune the size of isolated DNA by adjusting the agitation speed during lysis
Obtain best-in-class yields of highly pure and intact DNA, with efficient RNA removal
Experience excellent performance in long read sequencing
Available products for HMW DNA Extraction:
Monarch HMW DNA Extraction Kit for Cells & Blood
Monarch HMW DNA Extraction Kit for Tissue
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Monarch Mag Viral DNA/RNA Extraction Kit
The Monarch Mag Viral DNA/RNA Extraction Kit provides a rapid and reliable magnetic bead-based process for extracting viral nucleic acids from saliva and respiratory swab samples, and is compatible with wastewater.
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