Oregon Health and Sciences University
Available Kits for Plasmid Purification (Miniprep):
Monarch Plasmid Miniprep Kit
University of Massachusetts, Amherst
Total RNA Extraction
Sample type: Oat Protoplast Cells
Compared to: Qiagen RNeasy Plant Mini Kit
University of California, San Francisco
I often purify small amounts of inserts for ligations, so I appreciate the ability to elute in small volumes. I also very much appreciate the reduction of paper and plastic waste.
As with other Monarch kits, the design of the filter (no bezel) prevents ethanol retention, which can be an annoying problem with other kits. We've switched to NEB Monarch kits for all of our DNA purifications.
Available Kits for DNA Cleanup and Gel Extraction:
Monarch PCR & DNA Cleanup Kit (5 μg)
Monarch DNA Gel Extraction Kit
Researcher, UCL Great Ormond Street Institute of Child Health
Researcher, Iowa State University
DNA Cleanup and
Simple to use, good quantity and quality of purified DNA.
I can elute my DNA in a volume as small as 6 μl. I didn't have a lot of starting material to begin with so this helped concentrate my DNA too. I also ran a sample of my DNA after the clean-up for good measure and it was very clean!
Research Assistant, ND State University
Purify total RNA from multiple sample types (blood, cells, tissues and more) with a single kit
Includes DNase I and gDNA Removal Columns for efficient
Effectively purify RNA of all sizes, including small RNA (<200 nt)
Researcher, Yale University
Case Western Reserve University
Assistant Specialist, University of California Irvine
Sample type: Gram Positive Bacteria
Compared to: Qiagen RNeasy Mini
Natalie, PhD. Student, Winnipeg, CA
Mississippi State University
Available Kits for Genomic DNA Extraction and Purification:
Monarch Genomic DNA Purification Kit
Fast and easy to use, can get both genome DNA and viral DNA from virus infected cells.
This kit yielded the highest purity DNA I have ever seen from a commercial spin column kit.
I like the clear instructions and streamlined, small footprint packaging.
We have been using Qiagen for a very long time but when compared to the Monarch kit, we were surprised at how much more product we were able to collect. Great kits, easy to use protocols!! A+
It was easy to use and produce good quality, long DNA.
It works amazingly well for blood samples. I found the kit to be very easy and very quick to use. Probably the easiest protocol yet. The ability of this kit to do a single lysis step is great. It took me less than 20 minutes to go from sample to great quality DNA out of this prep.
Sample type: RNA from Virus in cell culture supernatant Compared to: TRIzol extraction
Assistant Professor, Haverford College
It was great for teaching.
My students knew when the solutions were mixed properly. They liked the color changes.
Less buffer retention as advertised! The indicator system prevents under and over-mixing for lysis and neutralization step, minimizing genomic DNA fragmentation.
Quickly and easily purify 20 µg of plasmid DNA from bacterial cultures
Eliminate buffer retention and elute in ≥30 µl
Track progress with our easy-to-use colored buffers
Research Scientist, OR Health and Science University
Plasmid Purification (Miniprep)
Jessica Purcell, Pirbright Institute, United Kingdom
Quickly purify DNA (50 bp to 25 kb) after enzymatic reactions and from agarose gels
Simple PCR cleanup in just 5 minutes
Elute in as little as ≥6 µl
Ensure no buffer carryover with unique column design
Purify oligos, ssDNA and small DNA fragments with a simple protocol modification
You don't have to wash multiple times with each wash buffer. Also, DNA recovery was very high and pure.
Kyle, Postdoc Researcher, University of CA, Berkeley
Purify RNA from various reactions including in vitro transcription (IVT), DNase I treatment, labeling and capping
Clean up and concentrate RNA after enzymatic reactions and after extraction protocols, including TRIzol®
Obtain clean RNA in minutes with fast and simple protocols
Select from 3 binding capacities: 10 µg, 50 µg and 500 µg
I compared the yield of RNA extracted and the purity of the RNA based on spectrometer (Nanodrop). When I compared to my usual RNeasy kit from Qiagen to the Monarch sample, I obtain 5 times more RNA from my sample as well as higher purity of my sample with the Monarch RNA mini-prep.
Principal Investigator, London, UK
Researcher,University of Massachusetts Medical School
I’ve had a go extracting RNA from a couple of different samples using the Monarch kit, in parallel with Trizol extractions from duplicate samples. The initial results, nanodrop analysis of the extracted RNA, were very positive.
I liked the consistency, ease of use, and the fact that everything you need (e.g. DNase, Proteinase K) comes in the kit. Both the concentration and quality of the isolated RNA from the Monarch Kit were slightly better than the GeneJet kit. The bigger difference was in the consistency of the RNA isolations between samples--it was much better with the Monarch Kit.
I've been using the RNeasy kit for some time, but I've been unable to have much luck. It seems geared toward eukaryotes and I've been working with Gram Positive prokaryotes… I tried the NEB [Total] RNA Miniprep Kit and it was much more straightforward. I also got excellent results the first time I used it.
DNA Cleanup and Gel Extraction
Elutions in small volume worked great, and allowed us to clone the fragment with no additional manipulations!
Sample type: CD34 cells
In our lab we routinely use gel purification kits. A major problem is inconsistency of sample recovery and less yield. I recently tried Monarch purification kit along with Promega kit on the same PCR samples using half of PCR reaction in each. And that was the "AHA moment" for me, With Monarch I got 65 ng/μl in total 25 μl of elution volume whereas Promega gave me 18 ng/μl in same volume. Totally satisfied, hope to get same consistent yield every time.
The Monarch kit was easy to use, the clever column design prevented EtOH carryover and allowed elution in a smaller volume. The increased DNA concentration is a real benefit for the multi-fragment ligations our lab routinely performs. Overall a great kit at a competitive price.
Sample type: Mouse Heart
Compared to: Thermo Fisher Scientific GeneJet RNA Isolation Kit
Genomic DNA Purification
Better yield and quality; easy protocol and good and consistent results among samples.
It is simply perfect!
Sample type: Yeast (fresh mycelium)
Compared to: Qiagen RNeasy Mini Kit
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What are you working with?
Easy to use and fast purification. Exponentially better than precipitation methods with great recovery.
Research Associate, Indiana University
OR Health and Sciences University
Sample Type: Leishmania donovani parasites
Available Kits for RNA Cleanup:
Monarch RNA Cleanup Kit (10 µg)
Monarch RNA Cleanup Kit (50 µg)
Monarch RNA Cleanup Kit (500 µg)
Purification of DNA and RNA is an essential step in many molecular biology workflows, including enzyme digests, transformation, electrophoresis, RT-PCR, RT-qPCR and library preparation for next gen sequencing. Monarch kits enable quick and easy purification of high-quality DNA and RNA, suitable for use in a variety of downstream applications. Recover pure, intact DNA and RNA in minutes with fast, user-friendly protocols and optimized buffer systems, and focus your time on the experiments that will drive your research forward.
I have been struggling to isolate enough product from the MinElute PCR cleanup and gel extraction kit for my PCR cloning project to be successful. We tried the Monarch PCR cleanup kit as a last-ditch effort and our ligation reaction was successful on the first try!
Professor, Ripon College
University of Massachusetts, Amherst
In comparison, the yield of DNA I'm getting from the Monarch kit is 5 to 10 times greater than with the Qiagen. Also, the quality of DNA is also better (based on Nanodrop absorbance curve). Finally, the Monarch kit is easier to use.
Your solution for nucleic acid purification
Total RNA Extraction and Purification
I loved the Monarch Kit. It is way better than the Qiagen due to the position and size of the filter in the columns.
Quality and quantity was OUTSTANDING!
Researcher, Walsh University
Purify high quality genomic DNA (gDNA) from a broad range
of sample types (cells, blood, tissues, and more)
Achieve excellent yields with extremely low (typically <1%) residual RNA contamination (RNase A is included)
Isolate high molecular weight gDNA (peak size typically
Take advantage of user-friendly protocols with fast and efficient lysis steps
I repeated the RNA extraction using [NEB’s Monarch Total RNA Miniprep Kit]. The quality of RNAs were checked using Tape Station. And all four samples had RIN between 8.3 to 9.3, so they are all good…Your RNA extraction kit is easy to use and saves time.
Researcher, Pacific Northwest Diabetes Research Institute
Researcher, Yale University
Sample type: Cultured cells
Available Kits for Total RNA Extraction and Purification:
Monarch Total RNA Miniprep Kit
Researcher, Lake Superior State University
Sample type: Tissue
Researcher, Yale University
Researcher, University of Strathclyde
Sample type: Bacteria
Staff Scientist, Minnesota
Sample type: Blood