Principal Investigator, London, UK
Elutions in small volume worked great, and allowed us to clone the fragment with no additional manipulations!
Quality and quantity was OUTSTANDING!
Quickly purify DNA (50 bp to 25 kb) after enzymatic reactions and from agarose gels
Simple PCR cleanup in just 5 minutes
Elute in as little as ≥6 µl
Ensure no buffer carryover with unique column design
Purify oligos, ssDNA and small DNA fragments with a simple protocol modification
Researcher, Yale University
Purification of DNA and RNA is an essential step in many molecular biology workflows, including enzyme digests, transformation, electrophoresis, RT-PCR, RT-qPCR and library preparation for next gen sequencing. Monarch kits enable quick and easy purification of high-quality DNA and RNA, suitable for use in a variety of downstream applications. Recover pure, intact DNA and RNA in minutes with fast, user-friendly protocols and optimized buffer systems, and focus your time on the experiments that will drive your research forward.
Purify total RNA from multiple sample types (blood, cells, tissues and more) with a single kit
Includes DNase I and gDNA Removal Columns for efficient
Effectively purify RNA of all sizes, including small RNA (<200 nt)
Mississippi State University
I loved the Monarch Kit. It is way better than the Qiagen due to the position and size of the filter in the columns.
Researcher, Iowa State University
The very rapid and easy protocol provided high quality DNA from difficult to lyse biological samples for lllumina and PacBio DNA sequencing. The use of environmentally friendly packaging was also a plus for me.
I can elute my DNA in a volume as small as 6 μl. I didn't have a lot of starting material to begin with so this helped concentrate my DNA too. I also ran a sample of my DNA after the clean-up for good measure and it was very clean!
Available Kits for Genomic DNA Extraction and Purification:
Monarch Genomic DNA Purification Kit
Professor, Ripon College
Request a Sample
Jessica Purcell, Pirbright Institute, United Kingdom
It is simply perfect!
Sample type: Oat Protoplast Cells
Compared to: Qiagen RNeasy Plant Mini Kit
Sample type: Gram Positive Bacteria
Compared to: Qiagen RNeasy Mini
In comparison, the yield of DNA I'm getting from the Monarch kit is 5 to 10 times greater than with the Qiagen. Also, the quality of DNA is also better (based on Nanodrop absorbance curve). Finally, the Monarch kit is easier to use.
I have been struggling to isolate enough product from the MinElute PCR cleanup and gel extraction kit for my PCR cloning project to be successful. We tried the Monarch PCR cleanup kit as a last-ditch effort and our ligation reaction was successful on the first try!
Barton Slatko, Senior Research Scientist, Genome Biology
In our lab we routinely use gel purification kits. A major problem is inconsistency of sample recovery and less yield. I recently tried Monarch purification kit along with Promega kit on the same PCR samples using half of PCR reaction in each. And that was the "AHA moment" for me, With Monarch I got 65 ng/μl in total 25 μl of elution volume whereas Promega gave me 18 ng/μl in same volume. Totally satisfied, hope to get same consistent yield every time.
Natalie, PhD. Student, Winnipeg, CA
Researcher, Walsh University
Sample type: RNA from Virus in cell culture supernatant Compared to: TRIzol extraction
Total RNA Extraction
Researcher, UCL Great Ormond Street Institute of Child Health
Kyle, Postdoc Researcher, University of CA, Berkeley
Oregon Health and Sciences University
DNA Cleanup and Gel Extraction
DNA Cleanup and
The Monarch kit was easy to use, the clever column design prevented EtOH carryover and allowed elution in a smaller volume. The increased DNA concentration is a real benefit for the multi-fragment ligations our lab routinely performs. Overall a great kit at a competitive price.
I often purify small amounts of inserts for ligations, so I appreciate the ability to elute in small volumes. I also very much appreciate the reduction of paper and plastic waste.
University of Massachusetts, Amherst
Plasmid Purification (Miniprep)
Researcher, Pacific Northwest Diabetes Research Institute
Quickly and easily purify 20 µg of plasmid DNA from bacterial cultures
Eliminate buffer retention and elute in ≥30 µl
Track progress with our easy-to-use colored buffers
I compared the yield of RNA extracted and the purity of the RNA based on spectrometer (Nanodrop). When I compared to my usual RNeasy kit from Qiagen to the Monarch sample, I obtain 5 times more RNA from my sample as well as higher purity of my sample with the Monarch RNA mini-prep.
Purify RNA from various reactions including in vitro transcription (IVT), DNase I treatment, labeling and capping
Clean up and concentrate RNA after enzymatic reactions and after extraction protocols, including TRIzol®
Obtain clean RNA in minutes with fast and simple protocols
Select from 3 binding capacities: 10 µg, 50 µg and 500 µg
It was great for teaching.
My students knew when the solutions were mixed properly. They liked the color changes.
I’ve had a go extracting RNA from a couple of different samples using the Monarch kit, in parallel with Trizol extractions from duplicate samples. The initial results, nanodrop analysis of the extracted RNA, were very positive.
Researcher,University of Massachusetts Medical School
Research Scientist, OR Health and Science University
Better yield and quality; easy protocol and good and consistent results among samples.
Available Kits for Plasmid Purification (Miniprep):
Monarch Plasmid Miniprep Kit
University of California, San Francisco
Available Kits for DNA Cleanup and Gel Extraction:
Monarch PCR & DNA Cleanup Kit (5 μg)
Monarch DNA Gel Extraction Kit
Simple to use, good quantity and quality of purified DNA.
Purify high quality genomic DNA (gDNA) from a broad range
of sample types (cells, blood, tissues, and more)
Achieve excellent yields with extremely low (typically <1%) residual RNA contamination (RNase A is included)
Isolate high molecular weight gDNA (peak size typically
Take advantage of user-friendly protocols with fast and efficient lysis steps
Your solution for nucleic acid purification
Researcher, Yale University
Total RNA Extraction and Purification
Research Assistant, ND State University
Assistant Specialist, University of California Irvine
We have been using Qiagen for a very long time but when compared to the Monarch kit, we were surprised at how much more product we were able to collect. Great kits, easy to use protocols!! A+
University of Massachusetts, Amherst
Easy to use and fast purification. Exponentially better than precipitation methods with great recovery.
Research Associate, Indiana University
I've been using the RNeasy kit for some time, but I've been unable to have much luck. It seems geared toward eukaryotes and I've been working with Gram Positive prokaryotes… I tried the NEB [Total] RNA Miniprep Kit and it was much more straightforward. I also got excellent results the first time I used it.
Assistant Professor, Haverford College
Available Kits for Total RNA Extraction and Purification:
Monarch Total RNA Miniprep Kit
What are you working with?
I like the clear instructions and streamlined, small footprint packaging.
Less buffer retention as advertised! The indicator system prevents under and over-mixing for lysis and neutralization step, minimizing genomic DNA fragmentation.
Case Western Reserve University
Available Kits for RNA Cleanup:
Monarch RNA Cleanup Kit (10 µg)
Monarch RNA Cleanup Kit (50 µg)
Monarch RNA Cleanup Kit (500 µg)
I repeated the RNA extraction using [NEB’s Monarch Total RNA Miniprep Kit]. The quality of RNAs were checked using Tape Station. And all four samples had RIN between 8.3 to 9.3, so they are all good…Your RNA extraction kit is easy to use and saves time.
Sample type: Yeast (fresh mycelium)
Compared to: Qiagen RNeasy Mini Kit
Sample type: CD34 cells
You don't have to wash multiple times with each wash buffer. Also, DNA recovery was very high and pure.
Sample type: Mouse Heart
Compared to: Thermo Fisher Scientific GeneJet RNA Isolation Kit
I liked the consistency, ease of use, and the fact that everything you need (e.g. DNase, Proteinase K) comes in the kit. Both the concentration and quality of the isolated RNA from the Monarch Kit were slightly better than the GeneJet kit. The bigger difference was in the consistency of the RNA isolations between samples--it was much better with the Monarch Kit.
Genomic DNA Purification