PCR
Mutagenesis
Direct Sample Amplification
NGS Library Amplification
ARTIC Viral Sequencing
Q5 is offered in standalone polymerase and master mix formats to support high-fidelity PCR applications.
Q5 Site-Directed Mutagenesis Kits enable rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.
Amplify a wide variety of targets direct from dried blood spots or up to 30% whole human blood.
Optimized for high-fidelity amplification of NGS libraries.
Use Q5 and LunaScript for fast and robust RT-PCR in ARTIC viral genome sequencing workflows.
PCR
NGS Library Amplification
Mutagenesis
ARTIC Viral Sequencing
Direct Sample Amplification
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Q5 High-Fidelity DNA Polymerase (NEB #M0491)
Q5 Hot Start High-Fidelity DNA Polymerase (NEB #M0493)
Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515)
Q5 High-Fidelity 2X Master Mix (NEB #M0492)
Q5 Hot Start High-Fidelity 2X Master Mix (NEB #M0494)
Q5 High-Fidelity PCR Kit (NEB #E0555)
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NEBNext Ultra™ II Q5 Master Mix (NEB #M0544)
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NEBNext Q5U® Master Mix
(NEB #M0597)
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LunaScript® Multiplex One-Step
RT-PCR Kit (NEB #E1555)
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LunaScript RT SuperMix
(NEB #M3010)
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Q5 Hot Start High-Fidelity
2X Master Mix (NEB #M0494)
ONE-STEP
TWO-STEP
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Q5 Blood Direct 2X Master Mix (NEB #M0500)
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Q5 Site-Directed Mutagenesis Kit (NEB #E0554)
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Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) (NEB #E0552)
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