It was great for teaching. My students knew when the solutions were mixed properly. They liked the color changes.
NEBNext Direct Custom Ready Panels have allowed my research to focus on the specific genes we need to explore. In addition to the convenience of easily selecting genes for focused panels, NEBNext Direct enrichment has provided the necessary reliability and depth of coverage to enable robust somatic variant calling.
NEBNext Direct enrichment technology is by far the fastest and most automation friendly protocol available today. I can have samples on the sequencer in 6 hours starting from genomic DNA. The technology produces very high on target percentages (>90%) for even very small panels, and in combination with molecular barcoding produces low duplication rates. From an optimization perspective, NEBNext Direct enrichment allows me to assign individual captured fragments to a probe unambiguously, thus giving the opportunity for optimizing the coverage distribution of any target.
NEB was fantastic while developing our panel or updating an existing one. The protocol is simple and fast and the results have been superb.
Simple to use, good quantity and quality of purified DNA.
We have been using Qiagen for a very long time but when compared to the Monarch kit, we were surprised at how much more product we were able to collect. Great kits, easy to use protocols!! A+
You don't have to wash multiple times with each wash buffer. Also, DNA recovery was very high and pure.
Researcher, Walsh University
Eric C. Olivares,
Professor, Ripon College
Dr. Guang Peng, M.D., Ph.D.,
MD Anderson Cancer Center
Luca Magnani, Ph.D, CRUK Fellow
Department of Surgery and Cancer
Imperial Centre for Translational and Experimental Medicine
Principal Investigator, London, UK
Researcher, Yale University