Heterogeneity across cancer types and within tumors challenges basic research, complicates disease management and impacts treatment efficacy. High-dimensional cell profiling of these microenvironments enables detailed analysis of cancer and immune cell maturation, activation and function associated with disease stage to thoroughly investigate heterogeneity.
Cancer
biology
The brain is the most complex organ in the body, composed of various types of cells including neurons, astrocytes, oligodendrocytes, glial cells and microglia that can be difficult to capture. Mass cytometry enables the identification, characterization and functional analysis of these cell types at the single-cell level, leveraging precise signal detection and a high detection capacity, no spectral overlap or background noise and reliable quantification with low cell input.
Neuroscience
Mechanisms and symptoms can vary between autoimmune diseases, but some core characteristics of autoimmunity tend to be shared. High-dimensional single-cell mass cytometry can be used to phenotypically characterize shared and diverging immunological features of autoimmune diseases, detailing the role of specific cell types and providing an overview of major immune cell populations.
AutoImmune
disorders
The ability to profile healthy and diseased samples using mass cytometry enables researchers to establish a single-cell atlas and evaluate distinct cell subpopulations that can be targeted to decrease inflammation. Data can also stratify patients based on relative proportions of inflammatory cells, providing the potential for precision therapeutic approaches.
Inflammation
The ability to customize treatments requires precise classification of cell subpopulations and simultaneous interrogation of phenotypic and functional properties of single cells before and after exposure to a therapy. Comprehensive analysis detailing an individual’s disease using mass cytometry enables investigation of cell populations and biological systems with unprecedented depth to provide superior outcomes for every patient.
Personalized medicine
Evaluation of treatment response using mass cytometry can distinguish responders from non-responders in large patient cohorts at the level of small or rare cell populations. This allows identification of actionable targets for novel therapies and prevention of disease relapse, and assists in increasing efficacy and safety of current therapies.
Therapeutic response
Immune monitoring is an important tool for studying the immune system’s role in the pathology, progression or resolution of disease. Mass cytometry provides thorough analysis of multiple cell populations simultaneously, especially for small samples such as limited patient biopsies in translational and clinical research.
Translational
immunology
Mass cytometry identifies immune cell populations with both breadth and depth, uncovering the immunological mechanisms of infection and vaccination. Rapid and reliable profiling of 50 or more phenotypic and functional markers in a single tube supports limited sample volumes and enables analysis of immune cells and signaling states at single-cell resolution.
Infectious
disease
