SALMON CASE STUDY

Objective: illuminate the epidemiology of piscine orthoreovirus-1 (PRV-1) in wild fish populations, particularly in endangered wild Pacific salmon


Utilization of Standard BioTools
products: 
• Biomark™ HD system 

• IFC Controller HX 

• 96.96 Dynamic Array™ IFCs

Conclusions:
 
• Atlantic salmon aquaculture may have facilitated PRV-1 spread from Europe to the North and Southeast Pacific 

• PRV-1 in endangered wild Pacific salmon may be fueled by aquacultural transmission, based on phylogeographic analyses and rtPCR surveillance

Objective: The objective of this study was to measure gene expression levels of Hemocyte immune response genes when exposed to White Spot Syndrome Virus (WSSV) and Fusarium solani (fungal) in order to understand the molecular mechanisms driving shrimp-pathogen interactions to develop strategies to control disease outbreaks in shrimp production systems with L. vannamei. 

Utilization of Standard BioTools products: 

Biomark System
NanoFlex 4-IFC Controller
96.96 dynamic arrays
Real-Time PCR Analysis Software 


Conclusions:
 
Genes that were monitored showed up regulation and down regulation over the time course study. 
Results reported can serve as immunomarkers for mortality events and could help reduce the loss in shrimp production systems

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Background

• PRV causes moderate to severe pathological changes in salmon,   endangering wild populations and resulting in downstream    effects in the ecosystem

• The introduction and origin of PRV-1 into wild salmon
   populations is unclear, thought to potentially be from farmed
   fish/aquaculture

• The authors analyzed viral genomes to help determine the
   evolutionary history of PRV and quantify transmission dynamics
   between farm and wild salmon populations

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SALMON CASE STUDY

Results and conclusions

Gene Expression and Epidemiology

Methods

Background

Results

Objective: The objective of this study was to measure gene expression levels of Hemocyte immune response genes when exposed to White Spot Syndrome Virus (WSSV) and Fusarium solani (fungal) in order to understand the molecular mechanisms driving shrimp-pathogen interactions to develop strategies to control disease outbreaks in shrimp production systems with L. vannamei. 

Utilization of Standard BioTools products: 

Biomark System
NanoFlex 4-IFC Controller
96.96 dynamic arrays
Real-Time PCR Analysis Software 


Conclusions:
 
Genes that were monitored showed up regulation and down regulation over the time course study. 
Results reported can serve as immunomarkers for mortality events and could help reduce the loss in shrimp production systems

Objective: illuminate the epidemiology of piscine orthoreovirus-1 (PRV-1) in wild fish populations, particularly in endangered wild Pacific salmon