Gene Expression and Growth
Schroeter, J.C. et al. "Development of a multitissue microfluidic array for assessing changes in gene expression associated with channel catfish appetite, growth, metabolism, and intestinal health." Aquaculture (2016)
Objective: develop and validate a high-throughput microfluidic array for assessing changes in gene expression associated with channel catfish appetite, growth, metabolism and intestinal health, while also providing a cost effective, dynamic gene expression platform for use with other cultured fishesUtilization of Standard BioTools™ products: • Biomark™ HD system
• IFC Controller MX
• 48.48 Dynamic Array™ IFCsConclusions: • Developed and validated channel catfish gene expression panel
• Provided reliable cycle threshold levels, amplification efficiency and quality threshold scores
• Demonstrated benefits of Standard BioTools high-throughput qPCR method over conventional qPCR
• Array paves the way for rapid evaluation of feeding strategies, dietary formulations, supplementation and environmental and management effects for improving channel catfish culture
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CATFISH CASE STUDY
Objective: The objective of this study was to measure gene expression levels of Hemocyte immune response genes when exposed to White Spot Syndrome Virus (WSSV) and Fusarium solani (fungal) in order to understand the molecular mechanisms driving shrimp-pathogen interactions to develop strategies to control disease outbreaks in shrimp production systems with L. vannamei. Utilization of Standard BioTools products: Biomark SystemNanoFlex 4-IFC Controller96.96 dynamic arraysReal-Time PCR Analysis Software Conclusions: Genes that were monitored showed up regulation and down regulation over the time course study. Results reported can serve as immunomarkers for mortality events and could help reduce the loss in shrimp production systems