Genotyping Using eDNA in Oysters
Holman, L.E. et al. "Demonstration of the use of environmental DNA for the non-invasive genotyping of a bivalve mollusk, the European flat oyster (Ostrea edulis)." Frontiers in Genetics (2019)
Objective: accurate SNP (single-nucleotide polymorphism) genotype information is
critical for a wide range of selective breeding applications in aquaculture, including
parentage assignment and marker-assisted and genomic selectionUtilization of Standard BioTools™ products: • D3™ Assay Design service
• EP1™ platform
• 96.96 Dynamic Array™ IFC for GenotypingConclusions: • Here we show that the collection of environmental DNA (eDNA) can be used to accurately genotype bivalve mollusks and potentially other aquatic organisms
• The influence of DNA extract dilution on genotyping accuracy was assessed to produce a practical protocol for the European flat oyster that can be used by researchers and aquaculture professionals as a template to develop viable alternatives to invasive tissue sampling in similar species
• We also demonstrated that eDNA extracted using this protocol is of sufficient quality and quantity for multi-locus genotyping, which is necessary for most applications in aquaculture breeding programs
TALK WITH US
OYSTER CASE STUDY
Objective: The objective of this study was to measure gene expression levels of Hemocyte immune response genes when exposed to White Spot Syndrome Virus (WSSV) and Fusarium solani (fungal) in order to understand the molecular mechanisms driving shrimp-pathogen interactions to develop strategies to control disease outbreaks in shrimp production systems with L. vannamei. Utilization of Standard BioTools products: Biomark SystemNanoFlex 4-IFC Controller96.96 dynamic arraysReal-Time PCR Analysis Software Conclusions: Genes that were monitored showed up regulation and down regulation over the time course study. Results reported can serve as immunomarkers for mortality events and could help reduce the loss in shrimp production systems