Gene Expression and Fish Welfare
Objective: examine how moderate hypoxia and incremental temperature increase affects transcript expression in the liver of post-smolts as compared to control conditions Utilization of Standard BioTools™ products: • Biomark™ HD system
• 96.96 Dynamic Array™ IFCs Conclusions: • Identified extensive transcriptional changes in stress and immune-related genes in the liver of post-smolt Atlantic salmon when exposed to temperature increase alone, or in combination with moderate hypoxia
• Identified several genes to be used as markers of stress response in post-smolt Atlantic salmon
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SALMON CASE STUDY
Objective: The objective of this study was to measure gene expression levels of Hemocyte immune response genes when exposed to White Spot Syndrome Virus (WSSV) and Fusarium solani (fungal) in order to understand the molecular mechanisms driving shrimp-pathogen interactions to develop strategies to control disease outbreaks in shrimp production systems with L. vannamei. Utilization of Standard BioTools products: Biomark System NanoFlex 4-IFC Controller 96.96 dynamic arrays Real-Time PCR Analysis Software Conclusions: Genes that were monitored showed up regulation and down regulation over the time course study. Results reported can serve as immunomarkers for mortality events and could help reduce the loss in shrimp production systems
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