Workflow
Flow cytometry workflow
Flow cytometry workflow
Imaging mass cytometry workflow
Imaging mass cytometry workflow
1.Collect
whole blood or isolate PMCs.
1.Section
tissue or tissue microarray onto a slide.
2.Stain
and process samples in ready-to-use assay tube.
2.Stain
slides with one antibody cocktail.
3.Fix
and freeze samples.
3.Ship
FFPE slides at room temperature.
Compatible sample types:
✓ Human- Fresh/Frozen
✓ Mouse-Fresh/Frozen
✓ Whole blood/PMCs/Cell lines
✓ Samples can be pre-stained and shipped or stained by Discovery Lab
Compatible sample types:
✓ Human- Fixed/Frozen
✓ Mouse-Fixed/Frozen
✓ Whole tissue sections/TMAs
✓ Tissue can be pre-stained and shipped or stained by Discovery Lab
4.Ship
frozen samples to any lab.
4.Acquire
selected ROIs or entire tissue on Hyperion XTi Imaging System.
5.Acquire
multiplexed samples on CyTOF XT
5.Validate
staining with MCD SmartViewer.
6.Report
population statistics, data plot displays and QC metrics with Maxpar® Pathsetter
6.Report
on spatial data analytics with Visiopharm® software.