PCR with the X9 System
1.Extract DNA from samples
(leaves or seeds)
NGS Library Prep
WorkGenomic analysis using microfluidics helps you minimize costs of material and labor while providing highly reproducible data from robust instrumentation. The X9 System offers a complete solution from low-density PCR to high-marker-density analysis needs for NGS.flows
PCR with the X9 System
Extract
DNA from samples (leaves or seeds).
2. Design PCR Panels and order using the free Standard BioTools D3™ service.
3. Add samples and assays to integrated fluidic circuit (IFC).
4. Perform qPCR and data acquisition using X9 system.
5. Analyze results.
2.Design GBS panels and order using the free Standard BioTools D3TM Service.
3.Add samples and assays to IFC.
4.Perform automated library preparation with X9 system.
5.Sequence on any Illumina® sequencer.
6.Analyze sequences.
2.Load RNA, reagents, oligo-dT beads on 48.AtlasTM IFC.
3.Perform automated RNA-seq library preparation with X9 System.
4.Harvest barcoded libraries and pool.
5.Sequence on any Illumina® sequencer.
6.Analyze sequences.
Automated library preparation enables the processing of many more samples and assays at one time compared with conventional methods, providing the confidence that quality libraries are generated without errors and requiring less manual interference.
NGS Library Prep