Cells are permeabilized and mixed with antibodies.
3. The aeorsol is passed through a heated spray chamber where it is exposed to a plasma torch, which vaporizes and ionizes the cells
5. The mass tags are then separated by their mass-to-charge ratio in a time-of-flight mass spectrometer.
4. The ion cloud is passed through a quadrupole to remove biological elements and isolate the mass tags.
6. Ion signals are collected per cell, resulting in single-cell mesasurements at subcellular resolution for downstream analysis.
7. Data is compiled in a standard FCS file format that can then be input into a variety of standard software.
2. The cell solution is placed into a nebulizer turning the mixture into an aerosol
