A sample containing cells suspended in solution is treated with a panel of antibodies, in which each is labeled with a different fluorescent dye
2. The cell suspension is introduced into a cytometer
3. Individual cells are registered as they pass through a set of lasers
Laser
Forward Scatter
4. The lasers excite the different dyes at different wavelengths followed by detection of light scattering and fluorescence emission
Side Scatter
Detectors
Color Filters
Dichromic Mirror
5. The data obtained from the flow cytometer can then be used to determine the cell types present in the sample and predict cell interactions and response to stimuli
